If yes, what is the ideal concentration of L-Arginine in DMEM?
Dear all I want to build a small fault model, considering the tectonic stress, the horizontal stress is relatively high.Is this the case where you can only use the stress boundary.This is a fixed...
13 July 2020 450 3 View
I would like to determine the structural changes of my protein at different temperature (eg. 350 K). In gromacs tutorial, the protein is equilibrated using nvt and npt under normal condition (300...
09 April 2019 9,392 3 View
05 July 2017 2,232 8 View
Hi, Is there a method to extract RNA of both gram positive and gram negative bacteria in their dual-species culture? I want to perform transcriptomic analysis, so I need RNA sample with high...
01 March 2017 7,922 2 View
I want to use SEM or other microscopy method to visualize the spectral organization of these two bacteria cultured together, however, they are both gram negative and rod-shaped. Is there any other...
08 March 2015 9,029 21 View
I found some protocols on the internet and I tried them out, but they don't seem to be working. I separated the cortex from the kidney and minced and then digested the tissue with HBSS buffer...
09 April 2014 9,313 1 View
01 January 1970 1,475 2 View
Deterium excess, or d, is defined by the following equation: d=delta(H-2)-8delta(O-18) what are the applications that have so far been reported in the literature?
01 January 1970 4,073 5 View
I'm working on Mucuna pruriens and want to quantify L-DOPA. Could someone please suggest the cheap and best solvent for the extraction and the solvent system that has to be used for its...
20 February 2021 6,058 2 View
Can anyone recommend a good code/software/implementation to identify the change of molecules (bond creation/break, number of molecules at a given timestep, etc.)? I am currently working with XYZ...
12 January 2021 1,655 4 View
Hello, I have a synthetic peptide with arginine residue I aim to methylate using PRMT1 + SAM (methyl donor). I then will use my dimethylated arginine peptide product in ELISAs and Western blots....
12 November 2020 5,568 7 View
Has anyone performed stimulation of fibroblasts to myofibroblasts in cell lines. I found some articles on primary cultures. does the same work with cell lines?
20 January 2020 5,969 4 View
Hi! I'm looking for a database, or similar, with information about different chemicals stability in water. Two examples that I'm interested in is L-carnitine and Trimethylglycine/Betaine, both...
31 December 2019 9,751 3 View
31 December 2019 8,481 4 View
I am trying to build a custom poly peptide that is ~50 amino acids in length, and it will ideally have a charge of ~+4 at pH 7. I can get the positively charged attributes from incorporating a...
08 December 2019 3,597 1 View
Hello, I am looking for tyrosinase enzyme inhibitors on B16F10 cells. Cuurently I am having problems for cellular tyrosinase activity measurement. Which I hav done successfully before. I dont...
10 November 2019 8,024 1 View
Hi. I want to do patch clamp on mouse oocyte. I have used poly L-lysine and either matrigel but no attachment was observed. Anybody knows another solution?
09 October 2019 2,371 3 View
Hello, We are working on refolding HLA A2 in the presence of a known HLA A2-binding peptide. Our alpha chain is about 80% pure and our B2m is >90% pure. After rapid dilution in the refolding...
09 October 2019 6,649 5 View
