If yes, what is the ideal concentration of L-Arginine in DMEM?
I transformed a strain of lactobacillus using electroporation to introduce a constitutive expression vector (P-erm is the promoter) expressing a recombinant protein. The construct was first...
29 November 2023 4,541 3 View
How to draw the molecular orbital diagram of copper oxide (CuO) and iron oxide (FeO)? I will need to determine whether the compound is paramagnetic of diamagnetic
03 August 2023 6,535 2 View
How to draw the molecular orbital diagram of copper oxide (CuO) and iron oxide (FeO)? Are there any good references? Thanks in advance.
03 August 2023 8,057 0 View
I encountered the problem of no signal detected from the flow analysis of my THP-1 derived dendritic cells (DCs). I have observed the morphology of my cells resembling that of DCs after...
05 June 2023 2,553 1 View
I would like to perform RAD-seq on one zooplankton with a body-size between 0.5-1cm. However, the DNA extracted from each individual by chelex can not work for library preparation. So I am...
02 June 2022 4,051 2 View
Just in the past summer, in Germany and other western European countries, once in 400 years large storms hit the region and caused casualties and significant property loss. Similarly in the...
28 August 2021 6,820 0 View
Hello everyone, I have conducted a multi-phenotype GWAS analysis. I have 5 phenotypes in total, the GWAS lambda ranged from 0.8 to 1.12, only two of them are acceptable (0.985, 1.030). Is there...
09 August 2021 6,306 0 View
I prepare in-house media in Duran glass bottles, and am trying to find a way to seal them to prevent any tampering. I currently use shrink plastics post-sterilisation (either autoclaving or filter...
09 June 2021 8,105 1 View
Dear all I want to build a small fault model, considering the tectonic stress, the horizontal stress is relatively high.Is this the case where you can only use the stress boundary.This is a fixed...
13 July 2020 557 3 View
Along the paleo-Tethys tectonic belt, the extention of the Himalayan geothermal belt, it would be interesting to investigate the origin of the high-T geothermal systems through comparison due to...
18 April 2020 2,671 0 View
Hi all. I ran the enzyme activity (pure Tyrosinase after purification using recombinant e coli) using L-Dopa with this condition 50 mM phosphate buffer ph 6, 1mM L-Dopa 25 C temperature 0.5 mM...
06 February 2024 8,350 4 View
Have anybody measured a kinetics of enzymatic digestion of BAEE(n-alpha-Benzoyl-Arginine Ethyl Ether) with USP method? I have observed acceptable delta-OD when use significant amounts of enzyme...
24 December 2023 9,368 1 View
We are currently having some problems with DAF2 or DAF-FM fluorescence. We use a 5µM concentration and are not able to see any effect of a stimulation on DAF fluoresnce. When looking cells under...
16 November 2023 8,182 0 View
I am using a magnetic separation based isolation kit for mouse neutrophils and the protocol mentions 2 incubation on ice for 15 min each. I am aware that low temperature pre-activate the...
05 September 2023 6,293 0 View
Greetings everyone. During my master's research, I focused on exploring the potential of a specific bacterial strain to produce antibacterial compounds. To achieve this, I used the technique of...
24 August 2023 588 4 View
I want to enrich the semen and soap vaginal sample in mycoplasma culture medium with horse serum, arginine and phenol red. But I have a problem with how to cultivate. After consuming arginine in...
27 July 2023 2,439 0 View
I am now doing the solid-phase peptide synthesis of the GnRH antagonist, ozarelix. Here are the amino acid sequences of it:...
18 July 2023 8,974 1 View
it means we have a set protocol of using poly L lysine for coverslip coating, according to our protocol after 4 our of incubation in 37 C ,we are used to dicard the poly L lysine ,but as the poly...
09 July 2023 5,335 0 View
what is the best source of Arginine for the synthesis of polyamines?
17 June 2023 7,877 1 View
I have L-Lysine powder from Sigma Aldrich. Based on MSDS, the L-Lysine powder can easily be solved with water, but in my case, some hydrophobic parts can't be solved. It influences the UV-Vis...
12 June 2023 4,664 5 View
