How to take p-xrd sodiated carbon anode material?

Can i collect p-xrd data of sodiated carbon anode of sodium ion battery in presence of air? Or I have to do the xrd in inert atmosphere?

23 January 2021 3,506 2 View

How to calculate the degradation rate in percentage ?

I performed the BCA assay to calculate protein content over the time period. However, I'm not understanding how I can calculate the degradation rate in percentage at each time point? What formula...

10 January 2021 9,094 3 View

How do moisture and air contribute to the capcity fading of fabricated anode materials for Na-ion batteries?

I am using a glove box with N2 supply for Na ion battery coin cell assembly. But the oxygen level is more than 20 ppm. Can anyone one suggest whether 20ppm oxygen level is okay for battery...

15 November 2020 5,727 3 View

Probe based primers for conventional PCR?

27 August 2020 1,935 2 View

While doing NIH quality assesement of cross sectional/ cohort studies ?

for e.g. lets say, out of 14 question, if we give 1 point to 3 question , 0 point to 1 study, other 10 questions are "not applicable" to this study. what is total score and what is conclusion

13 April 2020 7,574 2 View

Specific marker for neutrophil?

Hi, I will be carrying out flow cytometry using whole blood (human) and I'm looking for a marker that is specific to human neutrophils only. Any suggestions? Thank you.

08 March 2020 9,612 3 View

Does anyone know a specific marker for identifying eosinophils and basophils using flow cytometry?

I will be using human whole blood samples.

06 January 2020 3,767 3 View

Why peaks appear down in case of absorbance measurement in FTIR?

I tested the Titanium disc in FTIR after treating it with chemicals. But the absorbance peaks are showing down. I don't know what is wrong going on.

07 November 2019 1,963 5 View

Why is the UV absorbance spectra of dsDNA melting from my experiments not as per expectations?

17 April 2019 9,339 3 View

What should be recommended plating on Mild steel fasteners (M3-M8) to pass 200 hours of salt spray test without red rust as per ASTM B117?

What should be recommended plating on Mild steel fasteners (M3-M8) to pass 200 hours of salt spray test as per ASTM B117?

05 November 2018 3,899 2 View

Best solvent for L-DOPA extraction and HPLC analysis?

I'm working on Mucuna pruriens and want to quantify L-DOPA. Could someone please suggest the cheap and best solvent for the extraction and the solvent system that has to be used for its...

20 February 2021 6,058 2 View

Can I modify beginning of pelB/ompA tag as described?

Hello all, I am currently trying to modify my plasmids so that it has a sequence coding the pelB or ompA tag in front of the proteins. The problem is that in order to paste such a sequence into...

25 November 2020 8,386 3 View

Poly ICLC or Poly IC structure for docking ?

I want to do docking of Poly ICLC with protein. But, unfortunately there is no crystal structure for Poly ICLC or poly IC. Poly ICLC is a complex of Poly IC, poly lysine, and...

24 September 2020 6,107 1 View

Why this HPLC data show negative peak?

Lysine was dissolved in mobile phase and injected in HPLC but there are two peaks. One for positive at 2.1 min and another for negative at 2.8 min? Contents of moilbe phase are ACN:Water:TFA =...

08 July 2020 2,695 13 View

Fibroblast to myofibroblast?

Has anyone performed stimulation of fibroblasts to myofibroblasts in cell lines. I found some articles on primary cultures. does the same work with cell lines?

20 January 2020 5,969 4 View

How do I find the stability of chemicals disolved in water?

Hi! I'm looking for a database, or similar, with information about different chemicals stability in water. Two examples that I'm interested in is L-carnitine and Trimethylglycine/Betaine, both...

31 December 2019 9,751 3 View

Fibroblast to myofibroblast?

Has anyone performed stimulation of fibroblasts to myofibroblasts in cell lines. I found some articles on primary cultures. does the same work with cell lines?

31 December 2019 8,481 4 View

What is the best way to approach building a custom polypeptide?

I am trying to build a custom poly peptide that is ~50 amino acids in length, and it will ideally have a charge of ~+4 at pH 7. I can get the positively charged attributes from incorporating a...

08 December 2019 3,597 1 View

No tyrosinase enzmye activity determination?

Hello, I am looking for tyrosinase enzyme inhibitors on B16F10 cells. Cuurently I am having problems for cellular tyrosinase activity measurement. Which I hav done successfully before. I dont...

10 November 2019 8,024 1 View

Uneven poly-d-lysine coating/crystallization?

I've encountered problem with large but scarce PDL crystals and spotted coating after 1hr of 0.02mg/ml PDL in PBS incubation, PBS washed and dried. Previous coating from same PDL stock resulted in...

10 November 2019 5,272 2 View