Is it possible without using density gradient centrifugation?
You can choose certain protocol by following the links below to isolate yeast nuclei:-
1. http://plaza.ufl.edu/johnaris/Protocols/Yeast/YeastNuclei.pdf
2. http://www.biolchem.ucla.edu/labs/grunstein/Yeast%20Nuclei%20Isolation.html
3. http://jcb.rupress.org/content/49/2/423.full.pdf
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any fully funded phd position in wildlife ?
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