In my research i will collect saliva from pateints but the device for its collection is really expensive so what is the standardized method for collection of saliva by drooling method and what should be kept in mind during its transportation and handling?
Drooling method is best used for the collection of un-stimulated saliva. Its collected by using sterile cups (I had ordered for GC buffering capacity strips along with that I had got graduated sterile cups which was easy for collection as well as for recording the amount of saliva in a particular time). Commercially available salivary collection like OraSure allequets are pretty expensive. But you can use sterile cups with graduated markings that are routinely used in biochemistry setups. I am sure these will be available easily.
I would recommend to transport it to the respected lab within two hours of collection for further processing. If transportation time is more then you could use Liquid nitrogen for freezing the sample at -72Deg C and then transport.
Good luck for your research...
Dear Sadia Minhas
I agree with explanation given by Ritesh Srii Chettiar
Thank you Ritesh well what you think about Falcon graduated tubes for saliva collection is it ok?
- Dear friend for what purpose are you collecting saliva for? is it for biochemical analysis or a microbiological study? Are you keen in storing saliva for a longer time I mean transportation or something like that?
I will use saliva for virus detection through PCR
Secondly I will transport it to lab in maximum 3 hours
Dear Friend, I think the saliva can be stored up to 2hours with minimal changes happening, but I would prefer to use liquid nitrogen to freeze it and then transport it if it is more than two hours and also you are using the saliva for PCR study.
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