What is the protocol for human semen DNA isolation done manually?

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How to prepare Docetaxel solution for In vivo injections?

I have dissolved Docetaxel in DMSO to a concentration 0.5mg/ml, from which I take 1ul and dilute in saline (1:100) for mice injections. When I dilute docetaxel which is already dissolved...

25 January 2022 4,764 1 View

How can the data revolution be leveraged to advance more sustainable cities and transportation?

I am interested in examples of how the data revolution is already being used to drive more sustainable cities as well as ideas of new ways to leverage the data revolution for more sustainable and...

29 December 2018 726 3 View

May I know the shape of this particle based on the SEM image?

Need to know the shape of the nanoparticle.

22 May 2018 5,403 4 View

LHF is high in Arabian sea during DJF?

I have plotted climatology using tropflux data during 2014-16. It shows Arabian sea during DJF high.

24 April 2018 7,262 1 View

ROS effects on sperm Mitochondria?

what type of ROS and how its affecting sperm mitochondrial membrane related report by any factors ?

17 April 2018 9,660 3 View

How to identify the compounds present in the aqueous extract ?

I want to know the compounds present in my aqueous sample. Which analysis can predict that?

09 April 2018 8,956 6 View

How to form a fitness function using accuracy,error rate and computational overhead?

22 March 2017 8,887 4 View

Anyone knows how to prepare mitosox red dye by manual for mitochobdrial ROS analysis with required reagents ?

To measure the ROS from its origin mitochobdrial membrane

01 November 2016 3,842 1 View

Do you think 50gL-1 of PEG 6000 can cause osmotic stress to plants /poplar poplar? Please give me a nice reference

Those who have been doing research in Plant stress physiology, please answer.

25 July 2016 5,810 2 View

What software can be used to implement metaheuristic algorithm for feature selection in intrusion detection?

Read 2 answers by scientists to the question asked by Balasaraswathi Ranganathan on Feb 25, 2016

24 February 2016 9,720 1 View

How to learn more about SPSS and its Application?

I would like to learn more about SPSS and Its application especially in regards to data analysis. Please suggest me how I can learn more about it. Thank you so much.

11 August 2024 9,101 4 View

Can I base on reverse DNA sequences to perform alignment, convert to amino acids and GenBank submission?

I have reverse sequences (AB1 format), can I base on reverse DNA sequences to perform nucleotide alignment, convert nucleotides to amino acids and deposit the sequence in GenBank database?

11 August 2024 5,138 1 View

Baseline drift in HPLC? What causes this?

Hello, Why do i see this baseline drift when i compare my blank (black) to the sample (blue)? Any suggestions as to why this happened? Thank you!

11 August 2024 3,770 4 View

Text-Communication from the M1 Hand Area using BCI—and then there is Elon Musk?

Willett, Shenoy et al. (2021) have developed a brain computer interface (BCI) that used neural signal collected from the hand area of the motor cortex (area M1) of a paralyzed patient. The...

10 August 2024 7,180 0 View

Has anyone applied Python in the field of textile engineering for data analysis, automation, or smart textiles?

I'm currently exploring the application of Python in textile engineering, specifically in areas like data analysis, process automation, and the development of smart textiles. I'm interested in...

10 August 2024 7,429 2 View

How can I use the cif data obtained from rietveld refinement extracted via gsas2, for microstructural analysis using ETEX software?

09 August 2024 7,718 0 View

I can't see the ssDNA band after performing asymmetric PCR. Is there any way to do this?

After performing symmetric PCR, PCR purification was performed. Afterwards, asymmetric PCR was performed using the PCR purification product as a template, but no ssDNA band was confirmed in the...

08 August 2024 1,668 3 View

Does crude extraction using NaOH and Tris work well with Fungi?

I'm trying to find a DNA extraction method for fungi that does not require equipment and heating. Is there anyone who can suggest an alternative option? Thank you

08 August 2024 4,733 2 View

How are iso-frequency contours plotted?

Let's say we have a standard, regular hexagonal honeycomb with a 3-arm primitive unit cell (something like the figure attached; the figure is only representative and not drawn to scale). The...

07 August 2024 1,936 1 View

How to prepare the nanoparticle treated fungal sample for Environmental SEM analysis?

A fungal strain was treated with nanoparticles. We want to do an environmental SEM analysis. So could anyone share your views on preparing the sample? Thank you.

07 August 2024 5,307 1 View