For an insight about the type of phytochemical present, I will recommend you simply perform a simple qualitative analysis. You can even adopt preliminary chromatographical techniques such as Thin Layer Chromatochraphy or HPTLC or so. But if you are concerned about the specificiy of the phytochemicals such as what type of alkaloid or polyphenols and their respective quantities, I will recommend you perform an HPLC and Mass Spectrometry (GC-MS) analysis.
We covered this extensively in our book entitled Phytochemistry, Vol 1: Fundamentals, Methods, and Applications.
Your question was not very clear, but in general, the preferable method for analyzing aqueous extract of plant could be achieved via HPLC procedure. Possibly you can use different organic solvents to obtain more components. Good luck
Hi, I think the best way to predict the compounds in your aqueous extract is by using LC HR MS MS, after you get the data of molecule ion, molecule formula, MS1 and MS2, you can apply many online free MS data bases to predict the chemical structures. Best regards
For an insight about the type of phytochemical present, I will recommend you simply perform a simple qualitative analysis. You can even adopt preliminary chromatographical techniques such as Thin Layer Chromatochraphy or HPTLC or so. But if you are concerned about the specificiy of the phytochemicals such as what type of alkaloid or polyphenols and their respective quantities, I will recommend you perform an HPLC and Mass Spectrometry (GC-MS) analysis.
We covered this extensively in our book entitled Phytochemistry, Vol 1: Fundamentals, Methods, and Applications.