26 February 2017 11 6K Report

we have commercial metarhizium acridum powder (talc) formulation and we would extract spores/propagules to calculate germinated spores

we first tested salin solution containing; NACl/ KCl and tween 80 but it gives us nothing on PDA 

 either with simple distilled dionised water 

I used to extract fungi from commercial (powder) products using simple tween 20 and NaCl solution but this did not work either in this case

would it be possible that the product is not actif anymore (one year conserved at 4°C) ?

 Is there another protocol for that fungi ?

More Wafaa Mokhtari's questions See All
How to calculate mean and standard deviation using median and confidence interval?

Hello To perform meta-analysis, we need to know the mean and standard deviation in two intervention and control groups, but in the articles, Median and 95% confidence interval are reported (the...

28 April 2024 8,590 3 View

Is it possible to use dilution factor instead of concentration for crude?

Hello I have a cell free supernatant of bacteria and I want loaded this substances into nanovesicle. I want to know that it is possible to use dilution factor instead of concentration for...

14 January 2024 4,216 0 View

How important is learning and development in an organisation?

what is the importance of learning and development in an organization? How does this affect the employees and organization as well?

09 November 2023 594 5 View

Peptide detection method?

Hello I have a compound of small peptides(based on reference paper)and I can detect the peptide concentration. I don't know about details of it. I see the very good antimicrobial effect of it and...

20 October 2023 7,371 3 View

Ask a question related to the systematic review?

Dear Researchers I'm preparing a systematic review of case studies of a particular topic, and interestingly, although I thought there were many articles on this topic (the concept is very rich but...

19 August 2023 4,474 2 View

Peptide detection method?

Hello I have a solution and I want to know concentration of peptides I want to know I can draw standard curve with Nisin and then read kn 220nm absorbance? Or another way to detect peptides...

02 July 2023 5,848 0 View

Is there an experienced researcher in the field of water footprint to help me?

Dear Researchers, Please reply to my request, then I will explain what kind of assistance I need. Best regards

25 January 2023 4,059 4 View

Why are there always difficulties and challenges (racist) facing researchers in third-world countries in international publishing?

I think so

18 October 2022 8,028 8 View

What is Nisin prepration method?

Hello I want to do MIC test for staphylococcus aureus with Nisin A. I have a stock of Nisin with 68%potency. I prepared 1mg/ml concentration of nisin in deionized water. then make serial...

20 August 2022 5,104 1 View

Hi, I have had successfully run Trinity for de novo assembly by Trinity, but recently ran in to this error. How do I fix this error?

Left read files: $VAR1 = [ 'SRR14294419_1.fastq' ]; Right read files: $VAR1 = [ 'SRR14294419_2.fastq' ]; Saturday, June 18, 2022: 23:36:39 CMD: mkdir -p...

17 June 2022 2,488 0 View

Similar questions and discussions
Could it be a cell culture contamination?

Hello everyone! I observed in my culture (htert-RPE1 cells) an orange- red particle at the bottom of the dish. It is visible to the naked eye as a very very small red dot. Could it be a...

09 August 2024 2,824 3 View

How to isolate lymphocytes from mouse spleen?

I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...

04 August 2024 9,913 7 View

Does long-term culture of murine Monocytes result in macrophage differentiation?

Hi, I am isolating monocytes from the bone marrow using the Mouse Monocyte EasySep kit. I want to treat these cells and monitor expression of specific markers over the course of 10 days. I will...

04 August 2024 7,282 2 View

Good Day, can I ask for your help on the questionnaire that I want to adopt?

I am currently looking for a questionnaire that relates with my study which is The Effectiveness of Organizational Culture towards employee retention rates

03 August 2024 3,505 6 View

Can i quantify a nanoparticle encapsulated in cell culture medium by hplc?

Hello, if you made a transwell assay where you incubate the cells with a nanoparticle-encapsulated drug and considering that in the oposite compartment you'll have both the free and encapsulte...

03 August 2024 2,730 1 View

What is the problem with these tissue culture plants?

All plants are green but some of these plants becomes yellow. I did not found any reason. Please help me to find out the real problem.

01 August 2024 589 4 View

Found this object in the cell culture - do you have any suggestions what it might be?

Dear researchers, Today, we found this object in one of the wells of a 96wp culture plate. The plate contained MEC-1 cells and GelTrex. I am quite curious what this is (because it's not cells). Do...

01 August 2024 2,250 2 View

Is it common for heterotrophic bacteria to grow on Pro99 and SNAX media?

I am attempting to isolate picocyanobacteria from seawater (pre-filtered with a GF/D membrane) using the pour plate technique and filter plating method (Kearney et al., 2022) on Pro99 and SNAX...

30 July 2024 1,040 1 View

What exactly is it about biotechnology and medical research?

I'm very interested in biotechnology and medical research and I'd like to meet a professional here and talk about it together

28 July 2024 3,798 4 View

How does the Retention time effect the Biogas Production?

List down the various factors responsible and how does this effect the overall biogas production when it is related to Hydraulic retention time of the digester based on the feedstock?

27 July 2024 4,800 4 View

Our partners will collect data and use cookies for ad personalization and measurement. Learn how we and our ad partner Google, collect and use data. Agree & close