How does the performance of AgNPs vary when they are directly coated on a substrate vs when they are embedded in solid matrices before application?

Is the surface area and accessibility entirely different in these two cases? If yes, then, does that have a significant influence on the performance? Since my work pertains to antibiofouling...

07 December 2020 6,285 3 View

Is there any correlation between the chemical nature of coating agent and incorporated nanoparticles, in terms of performance, for surface coatings?

Generally, the inclusion of nanoparticles in paints and coatings is meant to uplift the properties of the latter. Is there any such report where the chemical nature of the coating agent might...

03 December 2020 1,545 7 View

How can i make my citation and H-index number, after 188 people read my research paper?

188 peoples have been read my Full length research papers, but till no citation and H- index number i found , please help me for making citation and H- index number, tell me the right way?

22 September 2020 9,577 14 View

Why do we soak anion exchange resin (I am using Amberlite resin XAD7HP (Sigma Aldrich)) in Ethanol- water prior to using it for adsorption purposes?

If the purpose of soaking anion exchange resin in water/ethanol is to open up its pores, then can we simply soak it directly in the sample being used for experiments(mine is plant extract in 50%...

21 November 2019 4,579 2 View

Wrong sequencing result after cloning?

I have cloned a Bacillus gene in E.Coli pET20b vector at EcoRI and HindIII sites. The vector was already having a gene cloned at the same restriction sites, therefore, before ligating the bacillus...

03 July 2019 9,340 4 View

How can we correlate two different data sets on different variables in spss?

There is one data set collected from teachers on principal leadership style (independent variable) and other data set is collected from students about their psychological wellbeing (dependent...

09 March 2019 7,064 4 View

How can we correlate two different data sets on different variables in spss?

There is one data set collected from teachers on principal leadership style (independent variable) and other data set is collected from students about their psychological wellbeing (dependent...

02 March 2019 4,369 4 View

Why there is no expression of recombinant protein?

I have cloned a gene encoding an enzyme from gram positive bacteria in E.coli BL21(DE3), tried all the IPTG conc. and temperatures but there is no sign of expression. Is there any incompatibility...

03 August 2018 6,509 8 View

How to map corresponding pixels from visible images to thermal images?

I have two sets of face images - RGB images, acquired from web-camera and Thermal images, collected using Optris PI450. I want to find the temperature of face regions like nose, forehead, chin,...

25 June 2018 2,698 4 View

How to know x,y coordinate values for commercially available blue emitting phosphor?

03 March 2017 5,484 3 View

Why does blocking with my immunogenic peptide cause nuclear fluorescence in ICC?

Hi all, I am validating a polyclonal antibody raised against human ANT4, through WB and ICC. In most of my controls, the antibody shows very little off-target binding, but when I pre-incubate the...

03 March 2021 5,999 3 View

Does Napabucasin soluble in dichloromethane and in ethanol?

I need to prepare a solution of Napabucasin (inhibitor of cancer cell stemness) in dichloromethane. The drug is expensive, so maybe somebody already tried to do it. If it is soluble, what is the...

02 March 2021 9,945 3 View

Help request in CST microwave?

im working now on TPV solar cells and metamaterials and i realy need our help in CST microwave i have a tpv cell simulation and i want to extract particullarly the absorbtion of only the active...

02 March 2021 2,246 2 View

Dapi fluorescence protocol for colon cell lines ?

28 February 2021 2,534 2 View

Which transition metal salt precursor is best for doping? Why?

I would like to synthesize a transition metal doped TiO2 via solgel method. Hence, I would like to know which metal salt precursor to choose among Chloride, Nitrate, and Sulphate. A clear...

27 February 2021 4,285 3 View

Does RNAiMAX transfection reagent work in serum free medium?

I diluted siRNA and RNAiMAX in opti-MEM and added to the cells which they were in the growth medium. Is it a right way? or should I culture cells in the opti-MEM medium for a while and not in...

26 February 2021 10,041 3 View

Has anyone had luck using cell lines other than HEK 293 (such as COS1) to package lentivirus?

I am looking to package lentivirus in endothelial cells but I am opposed to using 293 cells due to personal ethics regarding the source. I have done some reading and saw that COS1, COS7, & CHO...

25 February 2021 3,030 1 View

Unknown debris sheets observed in cell culture flasks?

Hi, We culture neural progenitor cells in T175 culture flasks coated with PLO-Laminin. We have successfully grown these cells with our protocol for months. Recently, we observed large sheets...

25 February 2021 3,443 4 View

How do I transfect a large plasmid into PC12 cells with nerve growth factor (NGF) receptor?

I have a large 16 kb plasmid, which I need to transfect into PC12 cells. Lipofectamin 2000 didn't work and with GFP alone the transfection rate is very low. I also tried the neon invitrogen...

25 February 2021 6,635 5 View

What is a good reagent for lentiviral plasmid transfection in HeLa cells?

I have been using Lipofectamine 2000 but was hoping there may be a better protocol or one for something like Lipofectamine PLUS reagent.

24 February 2021 1,633 4 View