Is there any literature on why rubidium chloride method of preparation of bacterial competent cells offers much better transformation efficiency than traditional CaCl2 method?
Dear Experts, I, Namrata Tharwani Gaurkhede, am pursuing my Ph.D. studies at VNIT, Nagpur under the guidance of Dr. V.S. Adane on SuDS as a tool for urban flood alleviation in case study area/s....
03 May 2024 9,670 6 View
I want to develop an RL-based traffic signal controller. Following are the relevant details. Training environment: Isolated intersection in Vissim 8.00 Language: python RL libraries: pytorch RL...
10 April 2024 9,417 0 View
I am trying to insert a His tag in my vector backbone that is of pCDNA.However I am getting wild type colonies of the vector after sequencing. I have used 50 ng of template for mutation PCR and...
28 February 2024 9,712 1 View
I am currently working with PacBio SMRT sequencing data acquired from various sequencer platforms, including Sequel I, Sequel II, and PacBio RS II. These platforms generate both Circular Consensus...
05 December 2023 8,309 0 View
MY sample is iron oxide doped with Zn and the lattice parameters I have determined from the Rietveld analysis. While the ionic radius of Zn is more than Fe, I found that compare to pristine...
11 July 2023 5,974 2 View
I have attached 3 photographs of the wavelength scan performed for silver nanomaterial (not washed or purified) synthesized by varying only the pH while keeping other factors constant. I want to...
21 March 2023 5,420 0 View
Can ImageJ or any such software be used to determine the area covered by fungal growth as in the image attached to this question? If yes, then how? I require this for antimicrobial assays. Kindly...
05 December 2022 1,940 5 View
I gave overnight culture from my DH5alpha glycerol stock in 5 ml LB and 5 ml LB+Kanamycin as control.There was no growth in the antibiotic added LB the next day(validating that my stock isn't...
28 October 2022 7,859 8 View
I am trying to do a Site directed mutagenesis and after DpN1 digestion,I transformed the PCR product in DH5 alpha cells and subsequently plated it on kanamycin containg agar plate since my plasmid...
24 October 2022 9,157 4 View
which process is better and easy
25 July 2022 2,799 2 View
I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...
07 August 2024 7,535 4 View
Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...
06 August 2024 641 2 View
i have sorted anti-NP specific plasma cells from bone marrow of C57BL/6 mice at certain times after immunization with variable counts and isolated total RNA using TRIZOL method for RT-PCR using...
05 August 2024 8,835 1 View
Hello, everyone. I have tried to determine carrier motilities of some materials, by Density Functional Theory, using Quantum ESPRESSO. There are a few methods to do it, like a package called...
04 August 2024 8,894 1 View
Hi, I am isolating monocytes from the bone marrow using the Mouse Monocyte EasySep kit. I want to treat these cells and monitor expression of specific markers over the course of 10 days. I will...
04 August 2024 7,282 2 View
I imagine the battery drives the graphite into a carbocation, then chloride is oxidized to donate the electron to the carbocation, but what pushes the lost electrons of chloride to be shared...
03 August 2024 5,384 0 View
I have used PE and PE-Dazzle 594 fluorochromes and have managed to get NK cells to properly show GranzymeB expression after 4 hr PMA/ionomycin stimulaton, but for some reason my CD8 cells in the...
01 August 2024 7,677 2 View
I'm fairly new to making homemade comp cells and have done so with some mild success. What is generally an acceptable way of resuspending comp cells during wash steps? I've been told to swirl the...
31 July 2024 8,309 0 View
What types of mask designs for metal deposition, to be used in a PVD system, are best suited for perovskite-based solar cells?
31 July 2024 4,835 3 View
Hi there! I recently started working on Lx-2 cells. When I thaw the cells, they start attaching within few hours but later in about 2 days they started rounding and dying. I used DMEM+2% FBS...
31 July 2024 3,893 0 View