Namrata Ganguli

4 Questions 4 Answers 0 Followers

Questions related from Namrata Ganguli

I am getting Wild type colonies after doing insertion mutation as per my sequencing results?

I am trying to insert a His tag in my vector backbone that is of pCDNA.However I am getting wild type colonies of the vector after sequencing. I have used 50 ng of template for mutation PCR and...

29 February 2024 9,698 1 View

What is the principle behind the action of rubidium chloride in preparation of ultra competent cells?

Is there any literature on why rubidium chloride method of preparation of bacterial competent cells offers much better transformation efficiency than traditional CaCl2 method?

18 November 2022 2,921 0 View

Contamination in DH5alpha competent cells?

I gave overnight culture from my DH5alpha glycerol stock in 5 ml LB and 5 ml LB+Kanamycin as control.There was no growth in the antibiotic added LB the next day(validating that my stock isn't...

29 October 2022 7,850 8 View

Bacterial colonies containing a different plasmid after transformation on antibiotic plate.How can the contamination occur during transformation?

I am trying to do a Site directed mutagenesis and after DpN1 digestion,I transformed the PCR product in DH5 alpha cells and subsequently plated it on kanamycin containg agar plate since my plasmid...

25 October 2022 9,148 4 View