How does the performance of AgNPs vary when they are directly coated on a substrate vs when they are embedded in solid matrices before application?

Is the surface area and accessibility entirely different in these two cases? If yes, then, does that have a significant influence on the performance? Since my work pertains to antibiofouling...

07 December 2020 6,285 3 View

Is there any correlation between the chemical nature of coating agent and incorporated nanoparticles, in terms of performance, for surface coatings?

Generally, the inclusion of nanoparticles in paints and coatings is meant to uplift the properties of the latter. Is there any such report where the chemical nature of the coating agent might...

03 December 2020 1,545 7 View

How can i make my citation and H-index number, after 188 people read my research paper?

188 peoples have been read my Full length research papers, but till no citation and H- index number i found , please help me for making citation and H- index number, tell me the right way?

22 September 2020 9,577 14 View

Why do we soak anion exchange resin (I am using Amberlite resin XAD7HP (Sigma Aldrich)) in Ethanol- water prior to using it for adsorption purposes?

If the purpose of soaking anion exchange resin in water/ethanol is to open up its pores, then can we simply soak it directly in the sample being used for experiments(mine is plant extract in 50%...

21 November 2019 4,579 2 View

Wrong sequencing result after cloning?

I have cloned a Bacillus gene in E.Coli pET20b vector at EcoRI and HindIII sites. The vector was already having a gene cloned at the same restriction sites, therefore, before ligating the bacillus...

03 July 2019 9,340 4 View

How can we correlate two different data sets on different variables in spss?

There is one data set collected from teachers on principal leadership style (independent variable) and other data set is collected from students about their psychological wellbeing (dependent...

09 March 2019 7,064 4 View

How can we correlate two different data sets on different variables in spss?

There is one data set collected from teachers on principal leadership style (independent variable) and other data set is collected from students about their psychological wellbeing (dependent...

02 March 2019 4,369 4 View

Why there is no expression of recombinant protein?

I have cloned a gene encoding an enzyme from gram positive bacteria in E.coli BL21(DE3), tried all the IPTG conc. and temperatures but there is no sign of expression. Is there any incompatibility...

03 August 2018 6,509 8 View

How to map corresponding pixels from visible images to thermal images?

I have two sets of face images - RGB images, acquired from web-camera and Thermal images, collected using Optris PI450. I want to find the temperature of face regions like nose, forehead, chin,...

25 June 2018 2,698 4 View

How to know x,y coordinate values for commercially available blue emitting phosphor?

03 March 2017 5,484 3 View

How do I increase the yield of my PCR product?

Hello, We would like to increase the yield of our PCR product. We are running a series of PCR reactions that is targeting ~1.1kb sequence. We begin each reaction with ~400pg of template DNA...

02 March 2021 4,029 3 View

New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before?

Question to you and THEM, the New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before? There appears to be a core problem for...

02 March 2021 3,024 2 View

Research on Supply Chain Management in Indian context?

Hi, I am planning to apply for the PhD degree in the Supply Chain Mgt. with specific area of "Cold Storage warehouses" during Pandemics and wars. Where lock downs and shut downs are frequent....

02 March 2021 285 2 View

PACYC PCR not working after several attempts. Would anyone have any suggestions?

So, I have been trying to run a pACYC PCR which will be used later on for a Gibson Assembly. However the PCR is not working. I have already tried gradient PCR and changing extension time; however...

02 March 2021 1,146 2 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View

How to address this issue in the analysis of Real-Time PCR results?

To dear Researchers, I was analyzing a series of concentration for estimation of Real-Time PCR efficiency. The concentration was 1:10. I used MS-excel to evaluate Slope. The result of slope was -8...

01 March 2021 8,683 4 View

No title

Does anyone have the experience of using Taq Man probes in the QIAGEN Rotar- Gene qPCR machine?

01 March 2021 5,311 1 View

Does any one can help me choose a reasonable basis set for intermediate metal ions interactions with guanine (i.e Fe2+, Mn2+, Cu2+ and exc.)?

Hello every body Does any one has any idea to help me choose a reasonable basis set for intermediate metal ions (i.e Fe2+, Mn2+, Cu2+ and exc.) interactions with guanine or other DNA organic...

01 March 2021 6,187 2 View

Anyone that can help with details in phosphopeptides enrichment?

Anyone know the amount of protein before enzymatic digestion for phosphopeptides enrichment of serume sample in Ti-IMAC? Has anyone ever use Ti-IMAC(the material is from Chinese company named...

28 February 2021 7,310 3 View

What could be some reasons why unstimulated monocytes are getting activated?

Im doing PBMC isolation -> CD14+ enrichment using magnetic beads -> stimulation setup. My negative control is just cells in cRPMI but they seem to get activated over and over again.

28 February 2021 7,883 3 View