I have read some papers on MSC isolation from human adipose tissue. DMEM and DMEM/F12 medium have been used for expansion. I need to know which type of DMEM (low/high glucose) is preferred for MSC culture.
Hello Shima Nazari
You may want to refer to the article attached below.
Article Conditions to improve expansion of human mesenchymal stem ce...
In the above paper, three media were used namely, DMEM with high glucose, DMEM with low glucose and α-MEM to check the difference in morphology and cell proliferation of MSCs. It was observed that during the fifth to seventh passages, the cells lose their morphology and proliferation potential on DMEM high glucose and α-MEM media. However, the cells expanded rapidly for up to 10 passages on DMEM low glucose. Therefore, DMEM (low glucose) was considered as an appropriate medium for the proliferation and expansion of MSCs.
Best.
Thank you very much Malcolm
The paper was really informative. according to this paper DMEM Low Glucose 10% FBS is the preferred medium compared to DMEM high glucose and alpha MEM.
I have noticed some people use DMEM/F12 for expansion of MSCs. How is the performance of this medium? Does anybody has any suggestions?
Using DMEM/F12 10% FBS media for expansion of ADSCs works very well. If you are isolating from adipose tissue you should use DMEM/F12 20% FBS media until the first passage then you can switch to DMEM/F12 10% FBS media for expansion. After isolation, the cells are in bad shape and the 20% FBS helps them recover and they will reach confluency fairly rapidly. The rate at which they reach confluency also depends on how many cells are initially seeded in the flask. Switching them back to 10% after the first passage will slow them down a bit and also you won't be wasting FBS. Also I would recommend not using the ADSCs past passage 6. The cell morphology starts to change although their differentiation potential seems to remain the same.
Good Luck!
DMEM-LG plus 10%FBS and 1% antibiotics is the best medium for expansion of Umbilical cord and Placental derived MSCs