Shima Nazari

4 Questions 6 Answers 0 Followers

Questions related from Shima Nazari

How do I design MTT for enaluation of growth factor effcts for fast growing cells?

Hello everyone I am trying to design an MTT experiment for an immortal adherent cell line with doubling time of 20 hours. The goal is to evaluate effects of certain growth factors and their...

14 February 2024 3,217 4 View

How does the electrospining process affect exosomes?

We are going to design tissue engineering scaffold using electrospining. I was wondering if we can directly add exosomes from stem cells to the polymer solution and then make exosome containing...

08 February 2024 6,557 0 View

What is the preferred medium for expansion of Mesenchymal Stem Cells?

I have read some papers on MSC isolation from human adipose tissue. DMEM and DMEM/F12 medium have been used for expansion. I need to know which type of DMEM (low/high glucose) is preferred for MSC...

06 February 2024 9,346 6 View

Is it possible to use high-Rox CYBR green qPCR master mix in rotor based systems?

Hello everyone I was wondering if I can use high-Rox CYBR green qPCR master mix in rotor based systems which does not originally need ROX for signal normalization. Those instruments do not read...

01 January 1970 1,440 0 View