It has already been demonstrated that cancer patients have higher concentratrions of miRNA21 compared to control, but how much miRNA21 you can normally find in healthy patients serum (M, mole)?
Thank you a lot!
Dear Maria,
since no standard protocol exists for blood sampling, internal or spike in control, RNA preparation and amplification, I would suggest to include your own healthy controls (sex and age matched), use exactly the same method and compare.
Best of luck
Boris
and you might want to consider exercise as an important parameter
https://www.physiology.org/doi/abs/10.1152/ajpendo.00138.2018?url_ver=Z39.88-2003&rfr_id=ori%3Arid%3Acrossref.org&rfr_dat=cr_pub%3Dpubmed
B
Thank you very much for your useful answer Dr @Schmitz, I'll do as you suggested!
Best regards,
Maria Laura
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