11 July 2017 2 2K Report

I am treating C2C12 mouse muscle cells with 0.2mM palmitic acid and I want to know generally how much free fatty acids enter muscle cells after a meal to give relevance to the concentration.

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What are the factors that causes the sample to have higher efficacy at lower concentration?

We conducted an antibacterial study of a plant extract. Varying concentrations of crude extract were subjected to microbroth dilution assay. The result showed that only the lowest concentration of...

31 July 2024 8,666 3 View

Beta galactosidase assay in milk?

I am planning to measure the the activity of beta galactosidase enzyme using a standard kit to detect glucose production. The kit reagent is a mixture including horseradish peroxidase and...

12 June 2024 7,331 1 View

How to conduct/interpret power analysis for binary GLMM within-subjects with 2x3 independent factors?

Dear community, I can't seem to find any clear information on power analyses work so I'm throwing my question out onto the internet hoping for answers. :) Study Details I am doing a...

19 May 2024 9,214 4 View

Does TSO- salt in a 5% NaHCO3 solution turn the solution into a dark green color?

Here's the brief: 1. I synthesized alkyl gallates (ethyl gallate, butyl gallate, and amyl gallate) using Fischer esterification. 2. I used TsOH instead of concentrated H2SO4 as the catalyst. 3....

03 May 2024 6,737 5 View

Help on Modified Nanopore Sequencing to do Sanger-like sequencing for PCR amplicon from bisulfite converted DNA?

Hi, I'm planning to do sequencing using Nanopore. The main idea is to substitute Sanger Sequencing that usually used to sequence PCR amplicon from BCD (Bisulfite Converted DNA) using nanopore...

23 April 2024 6,637 0 View

Lyophilization of leaves, stem bark, fruits?

Is it recommended to freeze dry harvested leaves, stem bark, and fruits prior to trituration and extraction? I'll use the extracts for phytochemical analysis & in vitro bioassays. Thank you...

31 March 2024 5,920 4 View

Are nature-based solutions relevant to building a resilient society in the face of drinking water scarcity?

Although there have been a number of initiatives, the shortage of drinking water is still a problem for some communities. In this context, we can see that people's need for water is increasing as...

26 February 2024 5,285 3 View

How to remove DCU and its remaining traces from Steglich esterification?

Firstly, the compounds that I want to synthesize are alkyl gallates (ethyl gallate, butyl gallate, and hexyl gallate). The overall reaction: Gallic Acid + Alcohols + (THF, DCC, DMAP, 0 Celsius)...

25 February 2024 2,181 3 View

Has anyone worked with Science Paths - reportedly a book writing service?

Hello everyone, I receive many emails about different journals and publishers inviting manuscripts (some predatory, some not); however, received something new. Someone from Science Paths sent an...

12 December 2023 1,819 0 View

Achieving the SDGs by 2030: myth or reality?

According to a report by the Sustainable Development Solutions Network (SDSN) published in 2023, only 18% of SDG targets will be met by 2030. Should we expect a new global agenda?

26 November 2023 7,921 3 View

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Hello What should be done to separate and identify organic acids in HPC when their RetTime is the same?Like oxalic acid with Propanoic Acid.or acids that have a very close RetTime.

07 August 2024 8,782 3 View

Determining the worth of a point improvement in Hamilton Depression Scale?

Dear readers, Thanks for your attention. I am wondering about the health economic problem of quantifying the value of interventions which a) prevent, b) improve symptom profile and c) ultimately...

05 August 2024 3,246 1 View

Conjugation of PEG-Amine to an Amino Acid Using EDC?

I am attempting to conjugate PEG to an amino acid at the C-terminus, for the purposes of producing nanoparticles. I have been told that PEG modified with amine groups can be used for this purpose,...

31 July 2024 2,033 1 View

Is it possible to use a safer acid instead of monochloroacetic acid to convert bacterial cellulose to carboxymethyl cellulose?

Hi, researchers, Is it possible to use a safer acid instead of monochloroacetic acid to convert bacterial cellulose to carboxymethyl cellulose?

29 July 2024 4,570 0 View

My cfDNA extraction protocol using silica beads works with plasma collected in COD SAGM bags but not with plasma collected in K2 EDTA tubes. Why so?

I am currently performing cell-free DNA (cfDNA) extraction from plasma using an in-house developed kit that employs silica-coated magnetic beads. My protocol works effectively with plasma obtained...

28 July 2024 3,559 0 View

What is the Principle of Cysteine-carbazole-sulfuric acid reaction with glucose?

I have been looking for the principle of Cysteine-carbazole-sulfuric acid reaction in research papers and books, but couldn't find it. This reaction is mainly used for the quantification of...

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How does the human microbiome influence health and disease, including implications for personalized medicine and microbiome-targeted therapies?

Probiotics related.

22 July 2024 1,707 2 View

How to prepare bacterial conditioned media to study the effect of bacterial secretome?

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17 July 2024 3,785 9 View

Does the bread-making process lead to a sufficient reduction in lectin activity of bean flour to guarantee food safety?

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Can sulfuric acid be used to prepare anthrone reagent when it has oxidized and changed color in reaction to light?

Sulfuric acid has changed color to yellow.

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