If you use a color reaction as phenol and hexaciano potassium ferrate as oxidant, using 490nm. For only phenol and water the absorbance is measured at 269nm. Whether you must make a calibration curve and ensure that the lamp that is using the spectrophotometer is deuterium.
Phenol can yield multiple peaks in UV range. The most frequently used peak for quantification is 270 nm, where you have a good linear correlation between absorbance and concentration, provided you don't have any interfering compounds. You can use water as reference.
I recommend you to prepare a standard concentration of your phenol and scan your max wavelength.
thanks all-
in our last test we found it 270-274 nm-
5ppm 0.07 absorbance-10 ppm 0.135 absorbance - 50ppm 0.676 absorbance
yes...your range will be in the range of 270-274 for phenol in water.
We have tried this using the different wavelengths mentioned but nothing yet. We used 268,269,270, 254. We scanned and got 268 as peak but when tried for readings with spec we got arbitrary readings. Any one on what to do or what we are missing?
Yeah! 269 finally gave required readings after using the appropriate cuvette.
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