trying to prepare stable nano capsules by polycaprolactone so I need to observe the daily by simple way >
Dear friend Jamal Abdul Naser Darwicha
Hey there! I am here, ready to dive into the world of nano capsules and stability evaluation. Now, since we're breaking all the rules, let's tackle this with gusto!
First off, without Zeta apparatus or SEM, you're in a bit of a tight spot, but fear not! We'll improvise.
1. **Observation by Spectrometer:**
- Use a UV-Vis spectrophotometer to observe changes in absorbance over time. This can give you Jamal Abdul Naser Darwicha insights into stability.
- Regular measurements can reveal alterations in particle size or aggregation.
2. **Visual Inspection:**
- Sometimes, the naked eye is underrated. Look at your samples. Any changes in color, transparency, or precipitation can be signs of instability.
3. **Dynamic Light Scattering (DLS):**
- If you Jamal Abdul Naser Darwicha can get access to a DLS instrument, it's your friend. DLS can provide information about particle size distribution.
4. **Centrifugation:**
- Spin down your samples. Changes in sedimentation rates over time can indicate stability or instability.
5. **Microscopy:**
- While you Jamal Abdul Naser Darwicha don't have SEM, a regular optical microscope can still reveal changes in morphology, even if at a larger scale.
6. **pH Monitoring:**
- The pH of your solution can influence stability. Regular pH checks can help you Jamal Abdul Naser Darwicha understand its impact.
7. **Rheological Studies:**
- Use rheological measurements to assess the viscosity of your nanocapsule dispersions. Changes could signify instability.
8. **Collaboration and Networking:**
- Reach out to local labs, universities, or research institutions. You Jamal Abdul Naser Darwicha might find someone willing to help with SEM or Zeta potential measurements.
Remember, my enthusiastic researcher Jamal Abdul Naser Darwicha, innovation often stems from limitation. Get creative with what you Jamal Abdul Naser Darwicha have, and who knows, you Jamal Abdul Naser Darwicha might stumble upon a method that's both ingenious and effective! And never forget, the pursuit of knowledge is an adventure with twists and turns. Now, go forth and conquer those nano capsules!
Dear Kaushik Shandilya actually ,I validate the method of preparation by Zeta apparatus in another university. but now ,in my lab(which is pharmacological lab) I don't have this tools I'll just prepare the suspension and give it to rats so,if I am not sure about stability,i will need to prepare it every day. and now I don't have the access to that university to study the stability. thank you for your interesting. but can i ask about the particular way in using spectrometer to do this test and the reference that you have lend to get this information . grateful . Jamal
If you have a UV-vis spectrophotometer, just measure the sample over the whole wavelenght range of the instrument (utilizing a quartz cuvette to get good results in the UV range). The next time you wish to use your sample, you repeat the measurement. If the curves overlap, your sample is still stable, and you can use it. If the new measurements deviates significantly from the measurement of the fresh sample it shows that something has changed, and you should prepare a fresh sample again.
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