Journal of Research in Humanities and Social Science. Is a real magazine?

Dear colleagues: I have received many emails from a magazine called ournal of Research in Humanities and Social Science. I have gone to the website of this journal, and I read "some" indexes but,...

13 December 2020 6,208 6 View

How can I connect a thermocouple to an oscilloscope?

I would like to measure temperature with a very high acquisition rate during a test that takes place in under 1ms. I was thinking of using a thermocouple and an oscilloscope. Which kind of signal...

26 October 2020 1,736 6 View

How efficient are HiTrap desalting columns for separation of proteins from free fluorescent labels?

I am looking for a reliable and fast method for separation of protein-fluorescent probe conjugates from the respective free fluorophores present in labeling reactions. HiTrap desalting columns...

06 September 2020 8,039 8 View

PhyloCode now a mediatic debate?

The recent official publication of PhyloCode has caught my attention, not for new arguments being presented for a system that has already been extensively discussed and, as I see it, whose...

04 July 2020 726 10 View

How to write a password automatically in a bash script?

I have a script that download multiples files from a server, but for each one it requires me to write a password. These are 1000, so it would be laborious to write 1000 times the password. How...

02 April 2020 852 2 View

How to define parametric modulators for multiple conditions in SPM?

Dear RG Community, I am performing an SPM analysis in which I've got 8 conditions plus the baseline in an event-related experiment. Each condition has 4 trials, totalling 32 trials per run. There...

02 April 2020 7,060 1 View

Alkali Resistance of SS316, Nickel and Hastelloy?

Does anyone know or may anyone assist me to find which of these materials (Stainless Steel 316, Nickel, or Hastelloy) is the most resistant to a highly concentrated NaOH solution (>27 mol/L)...

26 March 2020 6,875 6 View

Recommendations/tips on publishing datasets?

In my research about vibration of hermetic compressors, I'm creating a dataset that I intend to make it available once I publish the paper it refers to. Do you have any tips or recommendations on...

18 March 2020 7,803 5 View

Tools for ncRNA in bacteria?

Hello, I am trying to determine if a region present in a Streptomyces genome acts as a ncRNA that affects gene expression. Which are the best tools to analyze sequences for this? Thanks!

24 November 2019 5,270 3 View

In Limma package, why should I linearize my data in some analyses?

tl;dr: Why data linearization is applied in the identification o differentially expressed genes/proteins? I am new to data analysis of big data like proteomics. As far as I know, a simple t-test...

16 November 2019 8,441 4 View

No title

02 March 2021 3,060 3 View

New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before?

Question to you and THEM, the New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before? There appears to be a core problem for...

02 March 2021 3,024 2 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Could you please recommend some good journal that accept very long revie papers (approximately 6000+ words) in neurosurgery, cancer biology ?

Could you please recommend some good journal that accept very long revie papers (approximately 6000+ words) in neurosurgery, cancer biology ?

01 March 2021 8,087 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View

Where to find data on trees in tropical agroforestry?

Hi, I'm looking for data (mainly related to management: growth rate, canopy size, soil and climate preferences, etc.) about tropical trees used in tropical agroforestry. Have you ever heard about...

28 February 2021 7,356 8 View

What could be some reasons why unstimulated monocytes are getting activated?

Im doing PBMC isolation -> CD14+ enrichment using magnetic beads -> stimulation setup. My negative control is just cells in cRPMI but they seem to get activated over and over again.

28 February 2021 7,883 3 View