What is the most accepted definition of dysbiosis?

23 January 2025 0 4K Report

Is there an accepted definition of dysbiosis? There are different definitions and not all agree on them. Is there a one-fit-all, preferably referenced, definition? Thank you

Badges
Science topic

More Luigi Marongiu's questions See All

How to assess the osmotic power of a substance?

Hello, I suspect that a molecule I am using is causing bacteria to experience osmotic shock. What chemical properties should I look for to compare this capability with those of other substances?...

06 August 2024 977 1 View

How to stain bacterial surface for electron microscopy?

I would like to investigate the surface of bacteria using electron microscopy to assess changes in specific conditions. Is there an established protocol for that? Thank you.

15 July 2024 2,338 2 View

How to assess bacterial permeability?

Is there an established protocol to measure the permeability of bacterial membranes? Thank you

30 June 2024 6,614 3 View

How to immobilize bacteria without morphology alterations?

I have a set of bacteria that I stained with fluorescent dyes to check for viability. The protocol suggests to place 5 ul of the suspension on a slide, cover with a cover glass, and observe on a...

16 June 2024 9,382 1 View

How to transfect environment bacteria?

I would like to transfect bacteria with some plasmids. However, the bacteria are not competent but either isolated from the environment or purchased reference strains. Can I transfect these...

16 June 2024 9,384 6 View

How to describe statistical values in words?

After performing statistical analysis, there is the need to report the p-values in the text. Is there an accepted (published) stratification range? For instance, is 0.04 'slightly' significant?...

28 May 2024 7,724 3 View

How to assess if Kd values (constant of dissociation) are meaningful?

I used Microscale thermophoresis(MST, from Nanotemper) to determine the constant of dissociation (Kd) between a protein (20 nM) and a ligand (1:2 serial dilution from 50 μM to 1.5 nM). I ran the...

04 May 2024 2,066 0 View

How to stain live bacteria for real time tracking?

I would like to track in real time the growth of bacteria using fluorescent miscoscopy. I remember there was at least one plasmid that could be used: transfected bacteria would emit a red or green...

29 April 2024 7,095 3 View

What magnification for organoid imaging?

Hello, I would like to take some publication-quality pictures of organoids. What magnification shall I use? Do I need a 60x or is a 40x enough? Thank you

15 April 2024 8,471 1 View

How to sterilize pipette without autoclave?

Hello, I think I am using a 5 mL pipette that is contaminated with bacteria. Nothing serious, just the usual environmental microbes. The problem is that the tips for the 5 mL pipette are without...

07 April 2024 6,716 1 View

Comment ajoute-t-on un lien avec HAL sur ResearchGate ? / How do I add a link to HAL on ResearchGate?

Je voudrais donner l'accès à la version auteur de mes articles référencés sur ResearchGate avec le lien HAL. Comment faire ? Où indiquer le lien HAL ? I would like to give access to the author...

27 May 2024 2,668 3 View

Amylin in mice plasma, any values or kit?

Dear all, I wish to measure amylin in mice plasma of C57Bl/6 mice. However, I struggle to find information in the litterature that is precise. I only could obtain one data, referencing 0.7 pM in...

20 May 2024 9,163 0 View

Convert this reference to the apa referencing style?

[1] Joannes Paulus Tolentino Hernandez; “Network Diffusion And Technology Acceptance Of A Nurse Chatbot For Chronic Disease Self-Management Support: A Theoretical Perspective”, THE JOURNAL OF...

01 May 2024 9,940 2 View

How to answer the reviewer question for XPS TEST?

Our data: Binding energy of the C 1s peak corresponding to CAC/CAH bonds in adventitious carbon, set at 285.0 eV. Monochromatic Al Kα radiation (hν =1486.6 eV) is used with the anode power set to...

31 March 2024 4,978 5 View

What is the best way to reference?

The war in referencing. Choosing between manual referencing and referencing tool. Must researchers stop referencing manually?

25 March 2024 8,966 1 View

How to calculate equivalent of concentration ammonia?

I want to make 30 equivalents of concentrated NH4OH using a 30% wt aqueous NH3 solution, referencing the method below. I'm having trouble understanding how to produce equivalents of concentrated...

14 March 2024 6,160 0 View

Overview of Machine Learning. What are the opportunities it brings to developers? What threats does it pose to society?

I need a referenced cites on the above

17 February 2024 7,991 2 View

ReserchGate misatributes some citations to me. What should I do?

The site shows that my works are cited in some papers, however when I checked those papers, I found no sign of being referenced (on the other hand my work is referenced in completely different...

22 January 2024 3,766 2 View

Explain ISO 45001 in relation to safety and healthy management systems in the organization?

Explain ISO 45001 in relation to safety and healthy management systems in the organization? with intext referencing

28 October 2023 6,747 2 View

Can I please get a referenced methodology on how to assess the impact of mycorrhizal fungi on nutrients cycling and soil health?

I have an article describing a procedure but no citation in it.

21 October 2023 343 1 View