What is the minimum egg number per dilution I can use to determine EID50 for influenza viruses?

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Sagar Goyal

better to use at least 2 per dilution

Sabry Mohamed Tamam

4 ECE/ each dilution

Ilya Korotetskiy

No, you can't. I agree with Sabry Mohamed Tamam. You need use minimum of 4 eggs for each dilution. And only after that calculate the EID50 according to Reed and Muench

Quick Ida

I think 3 is the minimum. It is easier to get above 50% value with odd number of eggs.

Agnes Tinuke Laleye

Quick is right. The minimum number recommended by the OIE is 3 eggs per dilution.

Denys Muzyka

4 eggs for each dilution

Thomas Rowe

would use at least 3 eggs/dilution. If you expect a large concentration of virus in your samples, you can start your EID50's at a higher dilution

Sivasankar Panickan

5-10 eggs better

we perform a minimum of 3 eggs/dilution

Abdou Nagy

Minimum 3 eggs/dilution.