Punit is right - and it is any reduction that can cause color change - so every reaction that uses NAD/NADH/FAD will contribute to the color change.  This should only happen when cells are respiring - but it is well known that some bacteria - drug combinations produce purple, or "trailing", that can complicate the experiment.  It also means that you can't really tell the different between static and cidal readouts with one dye.

TTC is reduced to Tetrazolium, qhic is red in color. Reduction is done by bacteria. Same principle is followed in motility test by samusolid agar stab.

Thanks to all

Hi all, i am using the TTC to study bacteria viability too.. however, throughout the 14 days duration, the concentration of TPF obtain is fluctuated. Anyone of you can share your thought?

Thanks in advance

2-(4-IODOPHENYL)-3-(4-NITROPHENYL)-5-PHENYL-2H-TETRAZOLIUM also does the same reaction

Hi everybody. Can we use triphenyltetrazolium chloride as an indicator in MIC test instead of idonitrotetrazolium violet?

Thanks in advance of your co- thinking and guidance

Yes Fattahi, you can also use triphenyltetrazolium chloride

Akinyomade, thanks of your answer