What is the procedure for isolation of endophytic bacteria?

More Kalpna D. Rakholiya's questions See All

The residue of Frankincense resin still smells lovely after the essential oil extraction. Is it possible to make use of it for anything ?

It would be good to re-use bi-products where-ever possible so would appreciate any advice on left-over frankincense resin, after steam distillation to extract its essential oil. For example, can...

02 March 2018 6,440 4 View

For isolation of rhizospheric bacteria from halophytes generally which media is used?

for isolation of rhizosheric bactera from halophytes generally which media is used?

06 October 2015 4,454 2 View

What is the procedure for isolation of rhizoshere phosphate solublizing bacteria?

procedure for isolation of rhizoshere phosphate solublizing bacteria

20 September 2015 2,811 2 View

Which source is best for collection of cellulose degrading bacteria?

I am trying to isolate cellulose degrading bacteria but it isolates are not able to degrade cellulose potentially. So I need a suggestion which source is best and which method is best for...

08 September 2015 5,632 5 View

What is the mechanism of superoxide anion free radical scavenging assay?

what is the mechanism of superoxide anion free radical scavenging assay of plant extract?

24 May 2015 2,075 5 View

Which parameter and method should follow for Identifiaction, purifacation and characterization of crude extract?

I have acetone crude extract of medicinal plant for further study I need suggestion. After purification may used for antimicrobial and antioxidant activity.

06 May 2015 5,667 10 View

What is the procedure for Trichoderma production in agricultural point of view?

Procedure for Trichoderma production

02 March 2015 7,604 14 View

Which methods and sites are best for isolation of cellulose degrading bacteria?

I want to Isolate cellulose degrading bacteria for recycling Agricultural organic waste.

19 February 2015 3,212 3 View

Aromatherapy: Why is it that through our sense of smell, we can actually smell the "goodness" of an oil?

Pure high quality essential oils

13 November 2013 9,055 3 View

I have set up a new project in Research Gate entitled "Clinical Evidence of Essential Oils in Therapy". I would be grateful for any contributions.

Searching for research papers related to Aromatherapy / Essential oils - which demonstrate / indicate healing effects of essential oils.

06 October 2013 7,926 3 View

Is there any way to quantify bacterial and fungal cells in their mixed culture?

I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...

07 August 2024 7,535 4 View

What precautions should be taken while handling S. aureus enterotoxin Type B in the lab?

I would like to understand potential safety concerns while handling SEB in the lab. Especially while working in animal house facility. Would like to know precautions for handling. Sigma MSDS...

07 August 2024 6,034 3 View

How do soil microflora interact with plant roots and influence plant nutrition, health, and productivity?

06 August 2024 9,618 3 View

Weak DAPI staining after immunohistochemistry - how to improve?

After immunohistochemistry of previously fixed in PFA and EtOH and then frozen 20 μm sections of zebrafish brain, DAPI staining is very weak (right) compared to the same sections stained without...

05 August 2024 9,637 2 View

Do you have good tips for seaweed tissue preservation in the field for post RNA extraction?

I will be with my students collecting seaweed samples in a marine farm and later we will process this tissue for RNA isolation and further sequencing. Does anyone have tips on how to collect the...

04 August 2024 501 2 View

How to isolate lymphocytes from mouse spleen?

I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...

04 August 2024 9,913 7 View

Does anyone have issues using Prepman Ultra reagent for MicroSeq ID bacterial, fungal and yeast sample preparation?

I have been attempting to extract DNA from Bacterial, Fungal and Yeast banked samples (>1e7 cells) using Prepman Ultra reagent and I seem to be struggling to obtain a sequence. Although the...

01 August 2024 2,079 0 View

How is the bacterial genome's high protein count verified as genuine despite 800+ contigs and good metrics (98.55%completeness, 0.68% contamination)?

Given that the bacterial genome has over 800 contigs, but its quality metrics are good, with a completeness of 98.55% and a contamination of 0.68% as assessed by CheckM, what specific validation...

01 August 2024 1,514 1 View

Is it common for heterotrophic bacteria to grow on Pro99 and SNAX media?

I am attempting to isolate picocyanobacteria from seawater (pre-filtered with a GF/D membrane) using the pour plate technique and filter plating method (Kearney et al., 2022) on Pro99 and SNAX...

30 July 2024 1,040 1 View