I have acetone crude extract of medicinal plant for further study I need suggestion.
After purification may used for antimicrobial and antioxidant activity.
Check these links and publications:
https://www.researchgate.net/post/can_anyone_guide_on_how_best_to_quantify_the_phytochemicals_in_a_crude_plant_extract?_tpcectx=post_detail_recommendations&tpr_view=8f382a13-d93d-49b2-90a5-cc2db2cf5a61_2&_trid=849f61e9-e846-4464-bcef-b908f09fab5f_2
https://www.researchgate.net/post/Can_someone_list_out_the_methods_of_extraction_crude_extract_compounds_isolation_purification_identification_and_structural_elucidation?_tpcectx=profile_questions
https://www.researchgate.net/go.Deref.html?url=http%3A%2F%2Fwww.slideshare.net%2Frahulbs89%2Fextraction-of-plant-contituents
First you will need to extract the plant with different solvents sequentially using Hexane, Chloroform, Ethyl acetate and Methanol in that order. You will need DPPH (2,2-diphenyl-1-picrylhydrazyl) for the antioxidant assay. Do TLC for the fractions and spray with DPPH; spots on the TLC plate that turn yellow to the spray have antioxidant potential. Look up the book "Natural Product Isolation (Satyajit Sarker eds)" for a rigorous procedure. Thanks
Check this informative and interesting link:
http://www.slideshare.net/rahulbs89/extraction-of-plant-contituents
Following Jecinta advices I suggest you to try DPPH assays on any extract you prepare to know which one have positive reaction and then make the separation of the components on that extract through chromatography. This chromatographic separation provides you many fractions which have to be assayed again by DPPH until you get the more active fractions which have to be purified. To start from a crude extract to get the active compounds and it's chemical characterization really means many more steps and you have to go step by step. Good luck.
Good question. I agreed with J M Anowu and Olga lock.Any unexploited herb or plant, the first concern is to isolate and characterize the biochemical constituents, so wew can further study the use of particular constituents in food, medicinal or cosmetic products.
First of all try to find out the required activities of your crude extract , if it has the activities then go further for fractionation.
Kalpna am currently on this area of research, extraction, isolation and identification research are very demanding and expensive in countries like Nigeria that I reside, but I advice you do activity guided extraction first based on the polarity index of solvents. On the fractions obtained, YOU carried out antioxidant or antimicrobial assay, choose fraction with the highest activity for the isolation (Liquid chromatography, TLC and preparative TLC). For structure elucidation of your compounds, GC-MS, FTIR and NMR are suggested. Goodluck
Dear Kalpna D. Rakholiya,
GCMS gives only the volatile compounds,
You can not identify some unknown phytochemicals in the extracts only from FTIR and UV,
NMR is very complicated,
You can proceed with HPTLC and LC-MS,
Sincerely,
Gharavi
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