What is the procedure for isolation of rhizoshere phosphate solublizing bacteria?

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The residue of Frankincense resin still smells lovely after the essential oil extraction. Is it possible to make use of it for anything ?

It would be good to re-use bi-products where-ever possible so would appreciate any advice on left-over frankincense resin, after steam distillation to extract its essential oil. For example, can...

02 March 2018 6,440 4 View

For isolation of rhizospheric bacteria from halophytes generally which media is used?

for isolation of rhizosheric bactera from halophytes generally which media is used?

06 October 2015 4,454 2 View

Which source is best for collection of cellulose degrading bacteria?

I am trying to isolate cellulose degrading bacteria but it isolates are not able to degrade cellulose potentially. So I need a suggestion which source is best and which method is best for...

08 September 2015 5,632 5 View

What is the mechanism of superoxide anion free radical scavenging assay?

what is the mechanism of superoxide anion free radical scavenging assay of plant extract?

24 May 2015 2,075 5 View

What is the procedure for isolation of endophytic bacteria?

How we can distinguished endohytic bacteria and normal flora?

13 May 2015 2,587 4 View

Which parameter and method should follow for Identifiaction, purifacation and characterization of crude extract?

I have acetone crude extract of medicinal plant for further study I need suggestion. After purification may used for antimicrobial and antioxidant activity.

06 May 2015 5,667 10 View

What is the procedure for Trichoderma production in agricultural point of view?

Procedure for Trichoderma production

02 March 2015 7,604 14 View

Which methods and sites are best for isolation of cellulose degrading bacteria?

I want to Isolate cellulose degrading bacteria for recycling Agricultural organic waste.

19 February 2015 3,212 3 View

Aromatherapy: Why is it that through our sense of smell, we can actually smell the "goodness" of an oil?

Pure high quality essential oils

13 November 2013 9,055 3 View

I have set up a new project in Research Gate entitled "Clinical Evidence of Essential Oils in Therapy". I would be grateful for any contributions.

Searching for research papers related to Aromatherapy / Essential oils - which demonstrate / indicate healing effects of essential oils.

06 October 2013 7,926 3 View

What precautions should be taken while handling S. aureus enterotoxin Type B in the lab?

I would like to understand potential safety concerns while handling SEB in the lab. Especially while working in animal house facility. Would like to know precautions for handling. Sigma MSDS...

07 August 2024 6,034 3 View

How can I inoculate a lyophilized anaerobic bacteria into the culture medium without an anaerobic chamber?

There are anaerobic gas pak jars and environment providers in the laboratory that provide anaerobic environmental conditions for the growth of anaerobic bacteria. However, there is no anaerobic...

05 August 2024 4,945 3 View

Whether i can preserve leaves samples in 2.5% glutaraldehyde in 0.05 M phosphate buffer, pH 7 for coming several weeks for further LM study ?

I want to preserve the sample leaves for the LM study of the stomata and conidia analysis. Whether 2.5% glutaraldehyde in 0.05 M phosphate buffer with pH 7 can be applied for preservation.

29 July 2024 8,560 0 View

How does the Retention time effect the Biogas Production?

List down the various factors responsible and how does this effect the overall biogas production when it is related to Hydraulic retention time of the digester based on the feedstock?

27 July 2024 4,800 4 View

Question about MHB (Mycorrhiza Helper Bacteria) isolation technique??

What are the most effective isolation techniques for obtaining MHB?? Is there are best approach techniques? I keep struggling in obtaining it.

26 July 2024 7,387 0 View

Pink bacterial colonies?

I am cloning an overexpression plasmid with my protein of interest tagged with mScarlet. After transforming my ligated product into DH5α bacteria and plating on LB agar, I noticed colonies with a...

22 July 2024 5,953 6 View

What does negative readings in spectrophotometer denote. Was testing TSS content of bacterial cultures?

I was performing estimation of Total Soluble Sugar content in bacterial cultures by Anthrone method. Out of 9 samples, only one sample showed positive reading while others had a negative reading...

22 July 2024 9,576 4 View

How to prepare bacterial conditioned media to study the effect of bacterial secretome?

I would like to investigate the effect of bacterial secretome on cancer cells. How do you prepare conditioned media from bacteria?? conditions- time points - media I am looking for the best way...

17 July 2024 3,785 9 View

How do you decide an optimal AC amplitude for non-faradaic electrochemical impedance spectroscopy?

I work with planar interdigitated electrodes (IDEs). The finger width and the spacing between the fingers are 5 micron. The surface of the IDEs is coated by a non-conductive polymer layer...

16 July 2024 5,031 1 View

Can I use candle-jar technique to lactic acid bacteria?

I don't have the anaerobic generators (Anaeropac) so I'd like to try the candle-jar tecnique for the cultive of lactic acid bacteria

15 July 2024 8,890 2 View

Rahul Krishnan

you can go for selective isolation on Pikovskaya's broth and streak isolation on pikovskaya''s agar

C. Sarathambal

You try this media :Sperberg’s hydroxy apetite medium (Sperber, 1958). Serial diltuion and plating on these media and incubateat 30°C for 48 h. Observe the diameter of the clearing zones around the colonies