VDR gene in Echinococcus granulosus ?

In recent project we would like to evaluate the expression level of VDR gene in Echinococcus granulosus . For primer design we need that primer and the accession number of mRNA for this...

21 February 2021 8,673 1 View

How can extract RNA from plasma by trizol?

Hi Dear Colleagues, Could anyone explain an optimized protocol to extract total RNA from plasma samples? We would like to extract total RNA from plasma samples through Trizol, however, after the...

11 September 2020 7,456 3 View

Does anybody know any other visualization tool such as YellowBrick for Machine Learning visualization in Python?

I need a visualization tool similar to YellowBrick for the visualization of dataset and Machine Learning results in Python.

07 January 2019 2,503 2 View

What experiment type to choose for analysing TaqMan genotyping experiments with TaqMan Genotyper software?

Hi, I have assessed 4 SNPs by TaqMan genotyping assays from Applied Biosystems using StepOne plus realtime PCR system. Now, I tend to analyze my results via TaqMan genotyper software V 1.0.1....

17 September 2018 9,660 5 View

Fitting DFT calculated parameters in an EAM/alloy format?

Dear all, I have calculated necessary parameters including elastic constants, specific heat, equilibrium lattice constants, cohesive energies, migration and formation energies of defects and ......

21 November 2016 8,601 5 View

Zr-H and Zr-He eam/fs potential file for LAMMPS?

Greetings, A quick guide to access Zr-H and Zr-He (hydrogen and helium diffusion in Zr) eam/fs potentials suitable for LAMMPS will be appreciated. Regards Afshin

27 June 2016 7,872 10 View

DFT calculations of defect properties using Quantum Espresso?

Deal all, I want to calculate defect properties (i.e. formation energies and migration barriers, ...) in vanadium using DFT- Quantum Espresso, and I am wondering which of the available...

18 May 2016 9,850 3 View

Why does the Anti-Pyruvate Dehydrogenase E1-alpha subunit antibody give me an additional band on Western Blots?

20 September 2015 9,842 2 View

What are the best methods for the detection of macroautophagy?

23 December 2014 10,019 5 View

Are there any cell-based assays to detect the activation of all 3 arms of UPR simultaneously at the single cell level?

I want to detect the kinetics of the activation of IRE1, PERK and ATF6 at single cell level. Ideally cells expressing reporter constructs of equal efficiency to be able to detect temporal...

09 October 2014 5,076 3 View

I need to image mesoporous silica nanoparticles that are in ~100 nm size and do high resolution TEM to look at mesopores. what grids should I use?

Need to image mesoporous silica nanoparticles using the TEM. Also, need high resolution TEM images to see the mesoporous structure. Kindly suggest what kind of grids to use. Thanks, Shatadru

02 March 2021 1,787 2 View

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02 March 2021 3,060 3 View

What do you know about biotechnology art?

What do you know about biotechnology art?

02 March 2021 1,653 3 View

Which is the best place in the world for the plant biotechnology research?

I am searching for a good place for the Post Doc,

02 March 2021 4,053 3 View

Can chitosan alone can act as a reducing agent for Cu(0) nanoparticle sythesis?

[Synthesizing Cu nanoparticles without using chemical reductants]

01 March 2021 8,359 1 View

What are the new 2D nanomaterials were used for Silver nanoparticles stabilization?

In the stabilization of AgNPs are vital in current research interest. However, some of the 2D nano-materials like graphene oxide, MoS2, etc... previously used to stabilize it. But still they shown...

01 March 2021 2,207 3 View

What is the state of the drug inside solid lipid nanoparticles (SLNs)?

I am a little confuse with the mechanism of solid lipid nanoparticles (SLNs). The main point of encapsulating drugs into SLNs is because some drugs have low solubility in water. Therefore, SLNs...

01 March 2021 3,703 3 View

How to check the uv-vis absorption of nanoparticle by surface plasmon resonance?

I try to check the uv-vis absorption of Indium nanoparticle. Now I am measuring in solution, can I measure it in the form of a film?

01 March 2021 930 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Where to find data on trees in tropical agroforestry?

Hi, I'm looking for data (mainly related to management: growth rate, canopy size, soil and climate preferences, etc.) about tropical trees used in tropical agroforestry. Have you ever heard about...

28 February 2021 7,356 8 View