Hi
Dear Colleagues,
Could anyone explain an optimized protocol to extract total RNA from plasma samples? We would like to extract total RNA from plasma samples through Trizol, however, after the washing step with 75% cold ethanol, there are a high amount of reagent components such as phenol or carbohydrate (260/230 < 0.7). What do you suggest to get rid of these components?
Thanks in advance
#RNA_extraction #Tizol #Plasma #phenol or carbohydrate contamination
hello there
you can use less amount of reagent at the first step or high amount of isopropanol at the next step!!
I would like to suggest you to use column-based RNA purification kits if you want to have high quality RNA with the lowest possible contamination. The column removes any possible contaminations including phenol, Chloroform and DNA.
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