I can find a lot of information on what concentration you use to select for G418-resistance s. ceverisiae, however I can't find much on the concentration at which even resistant cells die.
For context I want to do a serial dilution of G418 and see what kind of range my G418-resistant (KanMX) cells are able to grow in, but I don't know the upper limit
Does anyone know from personal experience, or can show me a spot assay/ growth curves? I don't need exact concentrations - rough approximations are fine
Thanks
Hi there,
The level of resistance depends on the number of copies of resistance gene. Usual working concentration for resistant selection goes up to 2mg/mL for highly resistant clones. Usual concentration being 200 to 400µg/mL. But what is the point to work with such an high concentration where basically everything would be dead?
There is no fixed concentration that is guaranteed to kill all cells. You can titrate and go as high as 2-5 mg/ml but this is pointless for practical use.
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