What is the maximum concentration of imidazole that can be used to elute strongly bound protein from Ni-NTA column?

More Parismita Kalita's questions See All

Under what conditions absorption and excitation spectra of a same species can be different?

I took absorption and excitation spectra of a same species in the same solvent. Ideally I should get the excitation spectra almost similar to the absorbance spectra. But I got the excitation...

13 December 2020 8,965 2 View

Is it necessary that during peer review process the revised version of the manuscript is reviewed by the same reviewers who have reviewed it first?

During peer review process, as we all know we modify our articles based on the reviewers comment that we get. But when we submit our revised version of the manuscript, is it necessary that the...

29 June 2020 8,111 7 View

How do journal editors approach when multi reviewers submit conflicting decisions?

Suppose a paper have been reviewed by three different reviewer's. Two reviewer's suggested acceptance of the article, and one suggests not to accept the article. In such situations, what the...

02 May 2020 4,918 7 View

What are the Human Resource Practices of Indian Oil Corporation Limited (IOCL)?

I am researching for better Human Resource Practices which may be universally accepted by all organisations. Request all to share the answers/ links/ any documents/ videos

14 April 2019 6,418 3 View

How to perform SPAM steganalysis ?

I have downloaded the file from below link . http://dde.binghamton.edu/download/spam/ Thank you in advance.

17 September 2018 4,827 6 View

How does the electron mean free path or electron-lattice mean free path depend on temperature?

Mathematically, how does the electron mean free path or electron-lattice mean free path depend on temperature? Is there any formula linking the two?

12 December 2017 2,208 2 View

What will happen if we inject streptozotocin before nicotamide to induce diabetes in rats?

In general we induced nicotamide first which will be followed by streptozotocin after 15 mins to induced diabetes in rats. but i would like to know if we reverse the process. what about their...

09 December 2016 9,154 9 View

What should be the concentration of citrate buffer for anti-diabetic study in rat?

I will induce drug(Streptozotacin-Nicotamide) on rat as per the body weight. i want to know the concentration(molarity/normalioty) of citrate buffer ph-4.5, so that i can mix the drug and induce.

28 November 2016 8,240 3 View

Is there any method to compare two rank list and find out the better one?

Let's say I have two ranking list of homogeneous items. The rank list is prepared based on two different parameters, to find out the best items. Now is there any methodology that is helpful to do...

17 August 2016 5,420 3 View

Is it necessary to exclude stop codon in the primers sequence synthesized for clonning a gene into pET vectors with C-terminal His-tag?

I am using pET23(a) vector having a C-terminal His-tag to express a gene of 1.7kb. But as the reverse primer sequence includes stop codon , I think the his-tag expression is hindered. And...

16 March 2016 3,566 2 View

Buffer to disrupt platelet alpha granules for PF4 quantification?

Hello! I want to quantify by ELISA the secreted (from platelets poor plasma) and the non-secreted (from platelet lysate) PF4 before and after TRAP stimulation. I will use the ELISA from R&D...

03 March 2021 1,499 2 View

Buffer Exchange using column packed with sephadex G-25 effect on protein concentration?

Hello, If i am doing a buffer exchange for an antibody of 1mg/mL, does the elution lose protein in the process of buffer exchange? For example, if i flow through 500 uL of 1mg/mL sample, and...

03 March 2021 6,299 3 View

How optimize the Size Exclusion Chromatography?

Hello! I'll do a size exclusion chromatography, but I only have an open column, and I'll perform the peptide extraction from yeast, using buffer lysis (sodium phosphate 50 mM/NaCl 30 mM/DNAse and...

01 March 2021 2,215 2 View

Why are my bands weird in this Agarose gel?

Can someone please give me some possible things that could go wrong? Here is my recipe: 0.5g Agarose 50 mL of TAE 1x 1 uL ethyl bromide. Gel was run at 100V for 1 hour. The buffer used is also TAE.

01 March 2021 9,952 3 View

Why might my GFP tag be dissociating from my plasmid after transfection?

I have a set of stably transfected cell lines all transfected with plasmids containing GFP tags on the C terminus. During a western blot using anti-GFP antibody, one of my plasmids has dissociated...

01 March 2021 9,310 4 View

What do I do with DLS peaks that are from the buffer?

I'm looking at the aggregation of my protein sample using DLS. Unfortunately, my buffer (20mM HEPES) also results in a set of peaks. These are at approximately 1 and 1000 d.nm. The lowest peak...

01 March 2021 9,015 2 View

Precipitate after adding laemmli buffer to protein solution containing Potassium Acetate?

Hi, I am running a size exclusion chromatography experiment with a buffer containing Potassium Acetate as a salt. I analyse these fractions through SDS-PAGE. After boiling my SEC fractions in...

01 March 2021 2,622 3 View

What is the most suitable server for predicting protein structures in bacteria?

I have used the i-Tasser several times, however it has been unavailable for several days. I tried the swiss-model, but the output was not very pleasant due to the model used. Is there any other...

28 February 2021 4,521 3 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View

Are protein samples in SDS compatible with ELISA?

I have used an AllPrep DNA/RNA/Protein Mini Kit QIAGEN kit to extract RNA and protein from my samples. At the end of the protein purification, I resuspend my protein in 5% SDS. Will these samples...

28 February 2021 7,370 3 View