What is the maximum concentration of imidazole that can be used to elute strongly bound protein from Ni-NTA column?

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How to check leakage current of Si-SiO2 wafer?

I want to use the n-type Si wafer coated with a 300 nm SiO2 layer for the OFET device, but for that, I have to check the leakage current of the dielectric layer. how?

07 February 2024 4,115 2 View

What will be the quality of Al2O3 layer in OFET device fabricated through RF Sputtering?

This question is related to OFET devices. Please suggest some answers to this question.

07 February 2024 8,067 0 View

How to extract velocity data of a moving object from a simulation done in Ansys Fluent?

Hello Everyone!! I am currently working on a model for renewable energy harvesting using ocean wave energy in Ansys fluent. I have applied a dynamic mesh and compiled the UDF for motion of a...

30 January 2024 8,896 1 View

How to remove negative volume error in Ansys fluent dynamic mesh?

Hello Everyone!! I am currently working on a model for renewable energy harvesting using ocean wave energy in Ansys fluent. I have a design ready along with a UDF for a moving component. But when...

08 October 2023 5,531 5 View

How can we isolate exopolysaccharide content from cyanobacterial strains?

Is there any method for isolating exopolysaccharides (not the total Carbohydrate) from cyanobacteria?

06 August 2023 4,234 2 View

How can I find or estimate the species specific parameters α and β for application in Beta-poisson model for QMRA?

I am trying to estimate the QMRA for three species of bacteria using beta-poisson model. The literatures that I followed have not reported the α and β values for these 3 specific bacteria, while α...

27 May 2023 5,692 0 View

Q-Exactive calibration error for Mass resolution Dependency too high, can anyone help?

Hi, After performing my annual cleaning on the QE and calibrated, it as passes the positive cal but when performing the basic (negative) Analyzer accuracy it has failed for Mass resolution...

08 January 2023 2,206 1 View

What is the simplest method for hematocrit correction during glucose measurement in electrochemical dry strip technology?

Hematocrit correction during glucose measurement is very important for enhancing the accuracy of measurement in whole blood. As far my knowledge, it done electrochemically either through impedance...

20 December 2022 8,534 0 View

How to distinguish triplet-triplet annihilation band from phosphorescence and TADF?

If an organic molecular emitter shows multiple photoluminescence bands, what tools and techniques can one use to confirm the triplet-triplet annihilation (TTA) band?

18 November 2022 6,879 4 View

Can anyone tell me how to publish RFC standard?

I want to publish a work as an enhanced version of RFC 8180.

11 November 2022 6,235 1 View

AMAXA Lonza nucleofection lower volume of buffer?

Does anyone tried to do nucleofection with AMAXA by Lonza with lower than recommended amount of buffer in the cuvettes (100 ul)?

07 August 2024 4,616 0 View

Why I can't see any band in SDS-PAGE?

Currently, when I run SDS-PAGE, I don't see any bands at all, even though I used the same material just a day ago and it worked fine.... In our lab, we dilute the 10X running buffer to 1X and...

06 August 2024 5,373 2 View

Why does my protein refolded to beta sheet during thermal denaturation analysis?

Hi! So i attempted to understand a novel protein behavior towards heat application by analyzing its secondary structure change. I subjected the protein to a thermal denaturation analysis using...

06 August 2024 1,989 3 View

Why might the impedance values for DI water and 0.1X PBS buffer solution exhibit a decreasing and increasing trend, respectively over time (HP 4194A)?

Hello everyone, I'm encountering an issue with my electrochemical impedance spectroscopy (EIS) measurements and would appreciate some insights. Experimental Setup: Electrodes: Gold interdigitated...

05 August 2024 3,783 2 View

Low-yield gel extraction problem?

I am having an issue with my gel image where my PCR product is not appearing very bright on the gel. When I perform gel extraction, the A260/280 purity value is very low. I used the Qiagen gel...

05 August 2024 9,798 3 View

What is the best blank for nanodrop if I want to read a recombinant protein concentration?

Is it the "elution buffer" or the "dialysis buffer"? Note: I'll be using NanoDrop OneC

01 August 2024 967 3 View

Transfection in HEK293T cells?

Dear All, I am trying to transfect a pCDNA3.1 vector containing my gene of interest. The purpose is to figure out the localization of the protein of interest. I have fused the protein with GFP on...

31 July 2024 9,892 4 View

How to retain the a GFP tagged gene expression in stable cell line?

Hello , I established a stable cell line expressing GFP tagged to a centrosomal gene having G418 drug selection marker. I validated the stable line by IFA and Western blotting, results are fine....

29 July 2024 5,007 0 View

EDTA buffer solution?

Hello everyone! How can i prepare EDTA buffer solution (pH 8.2) to determine GSH levels? Thank you in advance.

29 July 2024 4,374 1 View

Whether i can preserve leaves samples in 2.5% glutaraldehyde in 0.05 M phosphate buffer, pH 7 for coming several weeks for further LM study ?

I want to preserve the sample leaves for the LM study of the stomata and conidia analysis. Whether 2.5% glutaraldehyde in 0.05 M phosphate buffer with pH 7 can be applied for preservation.

29 July 2024 8,560 0 View