Parismita Kalita

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Questions related from Parismita Kalita

What is the maximum concentration of imidazole that can be used to elute strongly bound protein from Ni-NTA column?

Lots of protein remains bound to the matrix even after elution with buffer containing 500mM Imidazole.

09 June 2016 4,959 2 View

Is it necessary to exclude stop codon in the primers sequence synthesized for clonning a gene into pET vectors with C-terminal His-tag?

I am using pET23(a) vector having a C-terminal His-tag to express a gene of 1.7kb. But as the reverse primer sequence includes stop codon , I think the his-tag expression is hindered. And...

17 March 2016 3,639 2 View

What is the role of glycogen in ethanol precipitation?

In a rRNA cleanup procedure for RNA-seq, use of glycogen(10mg/ml) was mentioned for purification of rRNA-depleted sample by ethanol precipitation. In routine practice we don't use glycogen...

28 October 2015 7,762 6 View