What is the major contaminant of extracted DNA from fresh plant tissues?

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Katie A Burnette

It depends on the tissue. If you are using seeds, then lipids or carbohydrates can be an issue. Some plants have lots of secondary metabolites that can interfere with DNA extraction. What kind of plant and what kind of tissue are you using?

Ayoob Obaid Alfalahi

Thanks Katie…

I am working on different plant types, like Sunflower, Soybean, Sorghum and Johnsongrass but I faced such problems especially with maize (Fresh leaves tissue).

Yuan-Yeu Yau

What kind of 'bad quality' did your isolated DNA have?

Did you grind the fresh tissue well enough? Becoming powder in the liquid N2?

Were your kit or CTAB solution old?