Do blood cells die after staining in blood smear analysis ?

This might be a very naive question but I've not been successful in finding sources that explicitly say that the cells are alive post staining. Do Romanowsky stains such as Giemsa, Wright etc....

09 September 2020 2,920 3 View

How and where do I get a reflective essay reviewed and published?

I spent the past few months interning as an independent researcher at the Field Museum in Chicago. Here, I spent my time observing the design procedure that leads up to the creation of a...

23 May 2020 770 10 View

How to design Model Predictive Control for Uncertain Nonlinear System with input delay?

Suppose we have a nonlinear system: x_dot=A x + B u(t-T) + phi(x) + d(t) y=Cx where x:state, u:control input, d:exogenous disturbance (bounded), phi(x): nonlinear vector (satisfy local...

24 June 2019 6,908 3 View

Can anyone answer the "Invalid cp problem at a particular temperature in fluent"? It will be a great help?

Invalid cp problem occurs in my fluent window. If I change cp of one material then same error occurs due to other. Is it a problem of mesh? Or any other problem. Please Help.

09 April 2019 1,977 3 View

Which AI platform is being used by Wireless Communication researchers for implementation of various ML/DL algorithms in their research?

I am more used to MATLAB, but I can't find suitable tutorials for advanced DL implementation in the context of Wireless Communications, using MATLAB. If you can suggest the answer, please let me...

21 March 2019 7,453 3 View

I have made a flexible i.e. less stiffness NFRPMC, How to achieve stiff composite?

Hello All, I have made a laminate which is very flexible which can be cut into parts by bare hands. I cured it at normal room temperature for day and half by placing load on laminate during...

08 June 2018 9,110 3 View

Are there studies on Indian climate history over the last 2000 years?

I'm attempting to build a hypothesis about the Gupta Empire of classical India, and am interested in understanding monsoon and temperature levels. Tree rings, Himalayan ice cores, any leads would...

18 March 2018 1,679 5 View

Can anyone inform me about some "rapid" peer-reviewed "SCI or SCI(E) indexed" journals in the field of Wireless Communications and related areas?

I need to publish some of my recent works in reputed journals but, unfortunately, I am short of time. So, I urgently need information about the aforementioned journals.

17 February 2018 1,985 4 View

Is there a specific model for gasification in fluent or do we have to use a UDF ?

I have performed combustion quite a few times but I am unable to do gasification. Is there a particular model in fluent or do we have to use a UDF. Do we specify products in species transport or...

21 January 2018 6,115 4 View

Can anyone suggest me any additional precautionary measures to be taken before thawing iPSCs?

Hi, I have recently thawed a vial of induced pluripotent stem cells on geltrex matrix membrane. I have used ready-to-use geltrex matrix solution to coat the well of a 6 well plate. Once a layer of...

20 December 2017 4,791 6 View

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02 March 2021 3,060 3 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

What is the most reliable source to get Impact factor of journals?

Source to get Impact factor of journals

01 March 2021 6,654 2 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View

What could be some reasons why unstimulated monocytes are getting activated?

Im doing PBMC isolation -> CD14+ enrichment using magnetic beads -> stimulation setup. My negative control is just cells in cRPMI but they seem to get activated over and over again.

28 February 2021 7,883 3 View

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28 February 2021 9,936 2 View

Which index or sites are reliable in determining of plant species?

There are many indexes when determining plant species: Plants of the World Online (POWO), International Plants Names Index (www.ipni.org), The Plant List (www.theplantlist.org) and others. But...

26 February 2021 3,404 10 View