12 Questions 24 Answers 0 Followers
Questions related from Anirudh Gangadhar
Hello, I'm working on a project requiring construction of a Deep Learning pipeline for multi-class classification using liver biopsy images. To start with, I would like to play around with some...
30 March 2023 5,867 3 View
After performing density-gradient separation, I am plating the obtained PBMCs with the intention to look at monocytes which adhere to the surface of the multi-well plate. A lot of protocols I...
09 November 2020 5,366 4 View
After separation from whole blood with a density-gradient medium such as Polymorphprep, two fractions are obtained: PBMCs and PMNs. We know that PBMCs contain Lymphocytes and Monocytes. Is it...
02 November 2020 535 4 View
Hi everyone, I use a density gradient medium, Polymorphprep to separate lymphocytes and neutrophils from human whole blood. In the literature, I have found that people do try to culture them in...
22 October 2020 4,370 3 View
Recently I performed RBC lysis to obtain WBCs from human whole blood. For my application, I use immunofluorescence to identify neutrophils and lymphocytes from this WBC population. The antibodies...
20 October 2020 5,456 5 View
The study I am working on requires isolation of lymphocytes and neutrophils from human blood. For the separation, I am using Polymorphprep. When I stain the neutrophil-rich PMN fraction, I do not...
04 October 2020 921 14 View
This might be a very naive question but I've not been successful in finding sources that explicitly say that the cells are alive post staining. Do Romanowsky stains such as Giemsa, Wright etc....
10 September 2020 3,161 3 View
I am looking to quantify the counting errors associated with serial dilutions. Few sources mention a lower cap of 25,000 cells per ml - does this sound right ?
28 December 2019 3,927 4 View
I face this problem while pumping bead solutions through a "hydrophilic" PDMS microfluidic device. Now since the surface was made hydrophilic and PDMS is hydrophobic, I'm thinking adhesion...
11 October 2019 6,832 3 View
While recording high speed images of MCF7 cells flowing in a microfluidic device, I've observed that some cells appear much brighter than the rest of the population. I've read that cells, just...
28 June 2018 2,056 2 View
I am working on some bead characterization studies and have tried to search for this: I've mostly come across flow-focusing techniques wherein one might produce uniform sized PDMS droplets and...
20 February 2018 6,179 0 View
Hi, I'm working on Circulating tumor cells (CTCs) and use Ficoll-Paque plus to isolate mononuclear cells (PBMCs) and CTCs from whole blood. My concern is that the low speed centrifugation done may...
01 January 1970 5,745 2 View
