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Questions related from Anirudh Gangadhar
After separation from whole blood with a density-gradient medium such as Polymorphprep, two fractions are obtained: PBMCs and PMNs. We know that PBMCs contain Lymphocytes and Monocytes. Is it...
02 November 2020 460 4 View
Hi everyone, I use a density gradient medium, Polymorphprep to separate lymphocytes and neutrophils from human whole blood. In the literature, I have found that people do try to culture them in...
22 October 2020 4,295 3 View
Recently I performed RBC lysis to obtain WBCs from human whole blood. For my application, I use immunofluorescence to identify neutrophils and lymphocytes from this WBC population. The antibodies...
20 October 2020 5,387 5 View
The study I am working on requires isolation of lymphocytes and neutrophils from human blood. For the separation, I am using Polymorphprep. When I stain the neutrophil-rich PMN fraction, I do not...
04 October 2020 844 14 View
This might be a very naive question but I've not been successful in finding sources that explicitly say that the cells are alive post staining. Do Romanowsky stains such as Giemsa, Wright etc....
10 September 2020 3,036 3 View
I am looking to quantify the counting errors associated with serial dilutions. Few sources mention a lower cap of 25,000 cells per ml - does this sound right ?
28 December 2019 3,811 4 View
While recording high speed images of MCF7 cells flowing in a microfluidic device, I've observed that some cells appear much brighter than the rest of the population. I've read that cells, just...
28 June 2018 1,940 2 View
