I am currently working on screening the Oxidative stress, glyoxal, methylglyoxal and advanced glycation-end products (AGEs) correlation with common diseases.
I found 2 main detection methods of AGES. First the spectrofluorimetry and second the ELISA to detect AGEs in general. I wonder if there is any other Profiling method to detect individual AGEs.
The state-of-the-art method to measure AGEs is liquid chromatography coupled with tandem mass spectrometric detection. It is outlined in this paper: "Quantitative screening of advanced glycation endproducts in cellular and extracellular proteins by tandem mass spectrometry" by Thornalley et al. (2003) Biochem. J.
http://www.ncbi.nlm.nih.gov/pubmed/12885296
This method seems to be very sensitive and accurate.
Best regards, Christian
HPLC with fluorescence detection could be used too, and allow the separation of several AGEs.
skin Advanced Glycation End Products by using the AGE-reader.... It is a better predictor of diabetic complications than HbA1c and predicts cardiovascular mortality
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