Abdullateef Nashed

9 Questions 8 Answers 0 Followers

Questions related from Abdullateef Nashed

Is there any effective and quick method to seperate peptides from proteins?

I am preparing a peptide from a fusion protein digest using a combination or proteases. The result mixture contains peptides range from 1-7 KDa and a acouple of proteins, all above 20 KDa. It...

13 June 2025 7,068 3 View

Is there a method or a published work on the comparison of a basal phenotype between two cell lines ?

I wonder if it is possible, in concept, to evaluate the basal phenotype of cell lines, and compare them. For example, If you want to compare the apoptosis potency of cell line A to that of cell...

13 November 2018 503 3 View

Cancer Prognosis and prediction markers ?

Hello. i am searching for well known (used in clinic) molecular markers with their criteria in breast cancer. i found many signature sets like, PAM50, MammaPrint. i wonder if there is reference of...

18 January 2017 7,437 3 View

'What is the most reliable database or source for cancer known/approved drugs' target?

Helloi am doing a survey on known and well validated drug targets in cancer for known drugs, any information will be appreciated

26 December 2016 3,098 2 View

BP Reaction in gateway system is not working, any suggestions?

Helloi am trying to construct an entry clone using gateway system. i designed the attb1,attb2 adopters to my primers and checked the purity of PCR product on gel. everything seems to be good. i am...

25 February 2016 8,746 4 View

How can I localize a protein, detected by 2D immunoblotting, on the silver staining gel?

I have been struggling in trying to localize the protein of interest i detect by 2D immunoblotting on duplicated silver staining.i tried to duplicate the 2 D gel and use one for 2D Immunoblotting...

09 June 2015 2,178 3 View

Any suggestion on organic extraction of indole's derivatives from aqueous solution ?

i am growing a bacteria in TB medium and i suspect presence of one coloured compound to be an indole's derivative. is there any recommended method to extract this compound in order to obtain...

09 March 2015 4,584 1 View

What is the latest up to date Advanced Glycation End Products (AGEs) detection methods?

I am currently working on screening the Oxidative stress, glyoxal, methylglyoxal and advanced glycation-end products (AGEs) correlation with common diseases. I found 2 main detection methods of...

28 January 2015 179 3 View

Is the Congo red plate a reliable method to roughly compare biofilm formation?

If not, what are the other methods? i am screening stationary phase phenotype differences between wild type and mutant E. coli and main phenotype was the growth difference (OD at the stationary...

27 November 2014 9,783 3 View