Lipid structures disappear upon negative stainging. Does anyone knows this effect?

13 December 2020 1,559 3 View

Cryo-EM Vitrobot settings for large lipid structures. How can I reduce ice thicknes?

Dear all, I have an issue figuring out good plunging conditions for large lipid structures: I have carbon coated C-Flat 2/2 400mesh copper grids with large (elongated) lipid structures on it....

13 December 2020 6,696 2 View

Can I use lentiviral packaging HEK293T cell lines to produce stable clones?

Hi, I generally made LV in HEK293T cell lines by triple transfection. I am wondering whether the newly generated LV will transduce the packaging HEK293T. In this way I could exploit the...

09 September 2020 6,333 3 View

Which software is the best for scientific presentations in which equations occupy a big part of it?

I usually use Beamer, in which equations are easy to write (and I already have them in Latex) but it's too static and rigid. In Powerpoint it is impossible to write the equations, it takes a lot...

15 July 2020 8,260 3 View

How to calculate the max Jsc for a given Eg using spectral irradiance [W/(m2*eV)] data?

I'm calculating the max available Jsc as a function of bandag Eg. For that I'm using the following integral: Jsc=q*int(f(Eph)*EQE.dEph) from Eg to \inf With: q=1C/eV f(Eph) Spectral Irradiance...

07 July 2020 5,873 4 View

Inverted current Mirror connection in LM741?

Analyzing the topology of the LM741 , the current mirror (Q8-Q9) that bias the first stage of the op. amp. is not connected as usual (see the red circle on the picture). Curiosly, this way to...

24 January 2020 8,419 2 View

Negative values in anti-inflammatory assay: inhibition of albumin denaturation?

I am performing in vitro anti-inflammatory assay: inhibiton of albumin denaturation by organic extract and purified fractions from natural material. Since my samples are colored (red, orange,...

14 September 2019 2,528 1 View

Can you help me with Python script?

Hi to all, I have an approaching deadline and to perform my analysis (design guide RNAs) I need to insert a lot of mutations in my sequenze (txt file). Mutations are inside a excel in file...

05 February 2019 2,494 1 View

What are good color palettes for students in the autism spectrum?

I've read that, for students in the spectrum, it may help to design a class environment that avoids overstimulating their cognitive system with unnecessary distractions, one of them begin colorful...

28 January 2019 2,557 3 View

How to improve undergraduate and postgraduate educational settings with gamification?

Gamification is a brilliant strategy to support the development of educational settings, especially in undergraduate and postgraduate courses. Therefore, I would like to ask you what do you think...

22 October 2018 2,456 3 View

Zwicky World Savior ?

03 April 2019 5,018 5 View

No title

30 July 2018 3,628 3 View

How to constrain dark energy model parameters using sneia when 1/H(z) function is not integrable?

the method of constraining parameters in dark energy models using data like SneIa requires finding integral of 1/H(z). This is because sneia data contains distance modulus data which we would like...

25 July 2018 1,982 2 View

Do Neutrinos Move At Light Speed And Have Mass?

"Ground-based telescopes, like the Anglo-Australian Observatory, saw the light from supernova 1987A several hours after the Kamiokande and IMB experiments had already detected the neutrinos that...

03 April 2018 3,689 1 View

What would be the best method for locating remnants of a type || supernova?

30 August 2016 6,554 4 View

What would be the best method for locating remnants of a type || supernova?

07 August 2016 4,461 4 View

Is it possible that SN1A and celestial objects generally lie in two cosmological reference frames?

SN1A are measured at different distances depending on whether redshift or luminosity is used. Might the situation instead be that the universe has two reference frames, so the same object is...

01 January 1970 6,904 7 View

Is the use of the Hubble diagram for dark energy valid?

Is it possible that there is a relationship for SN1A between luminosity distance and redshift distance that is different than the one assumed and graphed on a Hubble diagram?

01 January 1970 4,994 0 View

Does the universe include two reference frames, one static 3 dim space and the other dynamic changing 4 dim space?

Suppose there is a static 3 dim space and a dynamic changing 4 dim space. Without that supposition, lots of problems in physics remain puzzles. With it, lots of puzzles are easily resolved. Energy...

01 January 1970 7,875 2 View

How can the same SN1A light be at two different distances at the same time?

Using redshift, distance to a type 1A supernova can be estimated. Using standard candle luminosity, distance to a type 1A supernova can be estimated. Astronomical observation found luminosity of...

01 January 1970 9,171 2 View