what is the inhibitor for BH3 domain in Bax- Bid interaction?????
One with my previous position as Lecturer of Clinical Pathology in Fayoum University and the other with my current position as Associate Professor of Clinical Pathology in Fayoum University.
13 February 2024 3,713 1 View
I have a method using AOAC method to test dried fruits, vegetables and herbs for Total fats but it doesn't work for grains, such as oats, quinoa, breakfast cereal, etc. I get about half less than...
25 April 2022 287 16 View
I tried to cover the bitterness of the bioactive compounds of a medicinal plant by encapsulating them using the nanophytosome technique putting them in a sweet food product, but it did not work.....
30 December 2021 5,590 10 View
I extracted a plant rich in terpenoids with Supercritical CO2 (without any co-solvent) in the condition: ( vessel temperature=50 C, seperation temperature- 120 C, and pressure= 200 bar). I did not...
22 July 2021 2,599 2 View
I need to analyze sugars in freeze-dried food using GC-FID. I don't see any new techniques for this. I see this mainly done by HPLC-ELSD and RID. I've seen old technique whereby pyridine is...
09 June 2021 1,828 5 View
We are planing to do CRISPR but we found it is a little hard to do it in hiPSC at a moment. Can we apply it in cell line or not?
10 July 2019 1,950 2 View
I read about silver nanoparticles that they make the skin blue. But is that scientifically proven? What can cause that? And does that mean that they are toxic?
24 April 2018 2,611 3 View
I have to develop an amino acid method for only one amino acid and I need to derivatize but the HPLC does not have an on-line way to do it. How can I derivatize manually with OPA?
12 March 2018 6,104 4 View
Is Alibaba market real? are the prices including shipping? how to make sure that they will ship the goods and they will be in a good quality and manner and will not be late?
16 February 2018 6,908 5 View
Does anyone have papers on extraction of FAMES, omega 3 and omega 6 from seeds and herbs? I have to do this with a GC/MS or FID.
12 October 2017 7,252 2 View
Hi! So i attempted to understand a novel protein behavior towards heat application by analyzing its secondary structure change. I subjected the protein to a thermal denaturation analysis using...
06 August 2024 1,989 3 View
I aim to be as skeptical as possible regarding whether a pair of orthologous genes results in the same phenotype in their different but related bacterial organisms under similar environmental...
05 August 2024 6,787 4 View
I am performing ligation of the plasmid and a target gene. The steps I have taken are: 1. Double digestion of the plasmid and target gene 2. Ligation of the plasmid with the target gene 3....
05 August 2024 2,570 3 View
I have an RNA-seq data that I have analysed using Limma-voom and have extracted the gene IDs, log2FC and the p-values. At p value < 0.05, I have over 10,000 DEGs, however, when I run the GO...
31 July 2024 225 2 View
Dear All, I am trying to transfect a pCDNA3.1 vector containing my gene of interest. The purpose is to figure out the localization of the protein of interest. I have fused the protein with GFP on...
31 July 2024 9,892 4 View
I am currently working on a project involving liposomes and need to determine the maximum volume of siRNA that can be added to a 2.5 mL liposome solution with a total lipid concentration of 10...
30 July 2024 6,420 1 View
I am currently working on a project that involves extracting cell membranes, for which we disrupt the cells using sonication. During the initial extraction process, we add protease inhibitors to...
30 July 2024 7,077 1 View
Hello , I established a stable cell line expressing GFP tagged to a centrosomal gene having G418 drug selection marker. I validated the stable line by IFA and Western blotting, results are fine....
29 July 2024 5,007 0 View
I want to do 2,3-butanediol dehydrogenase(BDH) enzyme purification to confirm its activity for 2,3-butanediol. Before that, I need to confirm which N or C terminal tagging is better for enzyme...
28 July 2024 366 3 View
I cannot confirm cell surface protein expression by flow cytometry even after transfection and antibiotic selection of cells. Does it take long for proteins to express on the cell surface? the...
28 July 2024 3,178 3 View
