Can we apply CRISPR genome editing on cell line?

We are planing to do CRISPR but we found it is a little hard to do it in hiPSC at a moment. Can we apply it in cell line or not?

10 July 2019 1,866 2 View

Does anyone know how to use OPA as derivatizer for amino acidsif this can't be done on-line on HPLC ?

I have to develop an amino acid method for only one amino acid and I need to derivatize but the HPLC does not have an on-line way to do it. How can I derivatize manually with OPA?

12 March 2018 6,036 4 View

Urgent question : How I can keep Bax active ?

05 May 2016 5,031 2 View

Help on that bisulfite treatment protocol without using kit or restriction enzyme?

02 April 2016 9,942 1 View

I have problem to get a high density by using SW840 cell line?

08 March 2016 3,472 3 View

If I got a higher RNA concentration by Nanodrop, but nothing show up in Qubit. What does that mean?

If I got a higher RNA concentration by Nanodrop, but nothing show up in Qubit. What does that mean?

12 November 2015 1,146 5 View

Ideas for Phd's Thesis on pricing (Marketing)?

Hi everyone, I'm studying Marketing and I would like to write my PhD thesis on the topic of pricing. Any specific ideas?

02 March 2021 9,706 5 View

Is there any one who can provide a donation of HPV detection and genotyping kits in exchange collaboration forms?

Warm greetings! My name is Ayichew S. and I am doing my Ph.D. in Medical Microbiology focusing on Human papillomavirus). At this stage, I have almost completed a clinical sample (Cervical swabs)...

25 February 2021 3,612 3 View

Professional editing and proofreading services?

08 February 2021 7,901 6 View

How to calculate random forest manually?

17 January 2021 301 2 View

Is there and standard of Chemical price when writing a SCI paper?

Hello I writing a SCI paper and want to make a table for chemical price comparison. However, the chemical price varies from country and company, amount and also dates. Is there any reference...

12 January 2021 5,600 1 View

How do we solve optimization problems involving a maxima or minima in the objective function ?

I am trying to solve a multi-objective optimization problem using the Pyomo library in Python. The objective function includes operational optimization of an electrolyzer for hydrogen production...

11 January 2021 8,018 3 View

Can anyone identify this fungus/mushroom from China?

The pictures were taken in Guishan Mts in Yunnan. The fungus is ca. 10-12 cm high.

07 January 2021 6,639 10 View

Why nan loss values are resulted through deep learning in python?

hello every one i want to practice deep learning as descibed for a home price prediction problem i use code as following # IMPORTING PACKAGES import pandas as pd # data processing import numpy...

07 January 2021 8,761 1 View

Can you help with the implementation of two different model of deep learning for stock market prediction?

I have stock price data(Open, high, low, close), I want to do prediction by combining two different models like CNN with others. Dataset will be same, what are the ways that I can use for...

04 January 2021 1,845 3 View

How China is the biggest emitter of CO2 gas in the world?

How China is the biggest emitter of CO2 gas in the world?

25 December 2020 6,184 10 View