Can we apply CRISPR genome editing on cell line?

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I have two profiles in research gate with the same name Fadwa Abd El Reheem. How can I join both together to one profile?

One with my previous position as Lecturer of Clinical Pathology in Fayoum University and the other with my current position as Associate Professor of Clinical Pathology in Fayoum University.

13 February 2024 3,713 1 View

How to analyze Total fats from grains?

I have a method using AOAC method to test dried fruits, vegetables and herbs for Total fats but it doesn't work for grains, such as oats, quinoa, breakfast cereal, etc. I get about half less than...

25 April 2022 287 16 View

Which is the best nano-encapsulation technique to cover the bitter taste of the bio-active compounds?

I tried to cover the bitterness of the bioactive compounds of a medicinal plant by encapsulating them using the nanophytosome technique putting them in a sweet food product, but it did not work.....

30 December 2021 5,590 10 View

Which temperature is suitable for the micro-metering valve (separation valve) to extract terpenoids using supercritical CO2 extractor?

I extracted a plant rich in terpenoids with Supercritical CO2 (without any co-solvent) in the condition: ( vessel temperature=50 C, seperation temperature- 120 C, and pressure= 200 bar). I did not...

22 July 2021 2,599 2 View

How can I analyze sugars in freeze-dried food using GC-FID?

I need to analyze sugars in freeze-dried food using GC-FID. I don't see any new techniques for this. I see this mainly done by HPLC-ELSD and RID. I've seen old technique whereby pyridine is...

09 June 2021 1,828 5 View

What do you really think about silver nanoparticles? Are they safe to use them in food and food packaging? Or toxic to human?

I read about silver nanoparticles that they make the skin blue. But is that scientifically proven? What can cause that? And does that mean that they are toxic?

24 April 2018 2,611 3 View

Does anyone know how to use OPA as derivatizer for amino acidsif this can't be done on-line on HPLC ?

I have to develop an amino acid method for only one amino acid and I need to derivatize but the HPLC does not have an on-line way to do it. How can I derivatize manually with OPA?

12 March 2018 6,104 4 View

How can I use ( Alibaba )to buy something from china?

Is Alibaba market real? are the prices including shipping? how to make sure that they will ship the goods and they will be in a good quality and manner and will not be late?

16 February 2018 6,908 5 View

Does anyone have papers on extraction of FAMES, omega 3 and omega 6 from seeds and herbs using a GC/MS or FID?

Does anyone have papers on extraction of FAMES, omega 3 and omega 6 from seeds and herbs? I have to do this with a GC/MS or FID.

12 October 2017 7,252 2 View

Urgent question : How I can keep Bax active ?

I want to keep Bax ( pro apoptotic member) continuously active in mouse model. Can any one suggests me a method of that? Thanks

05 May 2016 5,153 2 View

Why Do TDS and EC Increase with Larger Wastewater Volumes, While BOD and COD Decrease?

I have carried out MFC experiments on three different volumes, 50, 500 and 1000 mL of wastewater. Results after MFC treatment shows that TDS and EC are more in larger volumes of water i.e. TDS and...

09 August 2024 9,621 0 View

Could it be a cell culture contamination?

Hello everyone! I observed in my culture (htert-RPE1 cells) an orange- red particle at the bottom of the dish. It is visible to the naked eye as a very very small red dot. Could it be a...

09 August 2024 2,824 3 View

Is there any way to quantify bacterial and fungal cells in their mixed culture?

I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...

07 August 2024 7,535 4 View

How to normalize and take the significance of the MTT OD values with 3 replicates for the same cell-line?

Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...

07 August 2024 8,106 4 View

Why activated CAR-Jurkat cell could not kill targets?

Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...

06 August 2024 641 2 View

How to isolate lymphocytes from mouse spleen?

I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...

04 August 2024 9,913 7 View

How to Add Missing Water Molecules in Protein-Membrane Simulations?

I have protein-membrane simulations (PDB, PSF, DCD) and have noticed that water molecules near the protein are not visible in the simulations. How can I fix this issue? Is there a way to place the...

04 August 2024 1,200 2 View

Does long-term culture of murine Monocytes result in macrophage differentiation?

Hi, I am isolating monocytes from the bone marrow using the Mouse Monocyte EasySep kit. I want to treat these cells and monitor expression of specific markers over the course of 10 days. I will...

04 August 2024 7,282 2 View

To perform transfection with DharmaFECT Duo in AGS cells. Could you tell me what the ideal concentration is to avoid significant cytotoxicity?

I would like to perform transfection with the reagent DharmaFECT Duo (Horizon) on the AGS cell line. Could you please inform me of the optimal concentration to use without causing cytotoxicity in...

03 August 2024 3,851 1 View

Who of all the Global Scientific community will help me Prof. Dr. Yoshida make way for TPEOM, MEC ~EMC to return the atmospheric gases to the norma ?

TEP presentation caption (The Environmental Project) Re: Why should Washington’s DC, or any country government point of location think of as nowadays of as to being 'tomorrow as to come! if it...

03 August 2024 2,484 1 View