Glucose simulation in GROMACS?

Hi all I am new to GROMACS and need help with some issues. (1) I am trying to run a simulation for glucose in water. The topology file for glucose was generated using AMBER and GLYCAM_06j-1...

04 May 2020 8,609 2 View

Purification of Octapeptin C4?

I am doing synthesis of Octapeptin C4 analogues with variable tail moieties as part of my project. I have made the final compound, which is cyclized and all BOCs removed. The problem is, I can't...

16 December 2019 1,730 2 View

Can you please provide me the sattelite data set for landcover change analysis of sundarban mangrove forest bd and india?

Can you please provide me the remote sensing sattelite data set for landcover change analysis of sundarban mangrove forest bd,india location ? If possible then send me the prepossessed dataset...

07 April 2019 3,794 3 View

What is the refractive index of benzyl alcohol?

what is the refractive index of benzyl alcohol 99%? i will measure the sample through zeta sizer but the solvent did not exit in the library of the machine?

11 February 2019 1,779 3 View

Can i reused sample measured by UV spectroscopy ?

my sample is small, can i reused the sample that already measured and get the uv on it???? for other test used can i??

10 February 2019 941 4 View

What solvent of ethanolamine dissolved in?

i try to prepare 3 %wt of ethnaolamine ? i will take 3 ml of 99% pure 98% ethanolamine ? what solvent used to put 3 ml in it?

04 February 2019 6,813 3 View

If the plant extract sensitive to temperature and can be degrade ?

i try to synthesis nano particles using green synthesis but the temperature surround 100 C and i notify the color change which indicate the nanopaticles formation but i read in the literature...

04 February 2019 5,110 6 View

What is the best live membrane stain for tracking zebrafish gastrulation?

I'm currently trying to live-stain the zebrafish embryo membranes during gastrulation, ideally beginning at 3 hpf until 6 hpf. However, I can't seem to find a good stain for the task. I have tried...

15 January 2019 4,320 3 View

Why their is no change in color?

i try to synthesize platinum nano particles from hexacholoplatinumic acid with plant extract? it should change the color after few minutes from pale yellow to brown ? but with no change in...

07 January 2019 7,346 10 View

What is better concentration in green synthesis of metallic nanoparticles of plant extract?

i have plant extract and i dried it and get mass in gram after evaporation the solvent, now i want to synthesize metallic nano particles but how much mass in g, mg should dissolve in which solvent...

22 December 2018 9,636 3 View

I have a problem with kanamycin - why the kanamycin doesn't affect?

Hi everyone, I need to expression of GFP with pET28 plasmid in E. coli BL21(DE3) plysE. Transformed cells have kanamycin and chloramphenicol resistance. Normally, only transformed cells should...

22 February 2021 4,032 4 View

Can 5-FOA Diffuse Through Agar?

I am preparing to do a gene knockout in the filamentous fungi Coccidioides posadasii targeting pyrG/URA3 so that 5-FOA can be used for selection pressure instead of traditional antibiotic...

16 February 2021 169 4 View

What are the naitve functions of bacterial NRP?

The bacterial NRPs (nonribosomal polypeptides) are famous as clinical drugs such as antibiotics, but is there other native function of these NRPs of bacteria except antimicrobial, because their...

01 February 2021 4,280 1 View

Why I am not getting cultures of DH5 alpha and BL21 cells after transformation?

Even if I get them cultured colonies are very less and almost not transferable and grows very slowly in the media. I checked cells by transforming with control vector. I changed antibiotic also...

06 January 2021 1,013 3 View

Is it normal to use Tris buffer when testing for bacteriostatic properties of isolated compounds?

Hello, to start off I attempting to test the bacteriostatic properties of certain compounds. I bought a kit that contains some of the equipment to perform this analysis, however, in the included...

02 January 2021 2,338 2 View

Is there any database or website from where I can get a list of FDA approved drugs for treatment of certain urinary tract infection?

I want to know all prescription drugs which are FDA approved compounds for infection like Urinary Tract Infections along with their causative agents. Can you guide me about any resource, database...

17 December 2020 4,852 3 View

Does anyone experience E coli lysis while seed culture?

Hi, I am currently working with BL21(DE3)PLySs and PRSET A vector. Out lab frequently transformation and culture E Coli for protein purification. But recently, even though there is colony after...

13 December 2020 9,167 9 View

How can i get nonsporulating bacterial bacillus subtillis strain spoIIE without antibiotic growth conditions ?

10 November 2020 7,168 4 View

Where can I find a database of antibiotics-dose required to kill bacteria?

Hi, I am Zaizhi. I would like to know where I can find a database of antibiotics-dose required to kill different bacteria. I found a few years ago. There, I was able to know the range of dose of...

08 November 2020 9,276 3 View

Which dose do you expose RAG1 deficient mice for lethal-irradiation?

Hello, I'd like to apologize for my English in advance, I'm still trying to polish it. We are performing several bone marrow transplantations (BMT) or fetal liver transplantations (FTL) to...

05 November 2020 4,239 1 View