The CLSI suggested the use of 0.9% PBS as a suspension medium for the AST test. I only have DPBS and i would like to compare the formulation of this one with the one in the CLSI to check I can use it.
I have tried to use this kit to measure released somatostatin from isolated mouse islets, but I had an issue where all measurements were low which should not be the case. doe any body have any...
24 June 2024 1,552 0 View
If any one did include any consent questions (few lines) in a tenant satisfaction survey. For example, we use de identified data from different housing providers tenants for benchmarking purposes....
19 May 2024 724 0 View
There is a lot of matrix effect that disable me to quantify it. what i need is a quick and fast extraction method. what should i add?
13 May 2024 6,902 2 View
How to prepare the sections and how to immunostain with three different antibodies? As I am going to use antibody to stain receptor of interest and anti insulin and antiglucagon. How can the three...
28 December 2023 7,154 0 View
Hi , I am looking for a full protocol for immunostaining of mouse pancreatic islets after isolation. I put them in RMPI after isolation. How can I prepare them for staining? and can I do...
05 October 2023 9,326 2 View
portable water quality assessment of some selected sachet waters pdf?
22 September 2023 1,744 2 View
I am going to do a radioligand competition binding assay, could any one help me with a protocol. The Kd value of my radioligand is 6.4. And the final volume of the assay is 500microliter, do I...
22 August 2023 2,236 0 View
Which method or modern technology, as a science instructor, will help raise the academic performance of students in secondary school? An innovative approach to teaching of abstract concepts...
20 July 2023 1,715 8 View
what is the cause of high scintillation counts in 35s GTP gamma s binding assay, despite of optimizing ligand concentration and all other parameters.
27 June 2023 1,234 0 View
How can preparation of sample for western blotting from isolated pancreatic islet be done?
23 June 2023 7,871 0 View
I have virus (viral hemorrhagic septicemia virus) in suspension and the experiment will not involve cells. What level of TCID50 is preferred?
11 August 2024 3,115 1 View
I am trying to adapt adherent CHO-K1 cells in F12K+10%FBS to suspension culture in only F12K, but in vain. I have followed Mirus bio as well as Thermo protocols, please suggest any alternative....
31 July 2024 3,756 0 View
I am working on natural remedies for de-worming in small ruminant livestocks. Part of my project involves formulation of drench-type product, which I have not much experience in. My experience is...
30 July 2024 9,806 1 View
While doing AST for Pseudomonas aeruginosa, after incubation, no zone of inhibition observed in the plate near the well. wells surrounded by bacterial growth, when the same plate observed under UV...
25 July 2024 9,229 1 View
I am working in the filed of long acting drugs with Nano formulation. I want to study the efficacy of my formulation in 2D cell cultures while this required media change on every two days. In such...
24 July 2024 8,696 0 View
"Dear Researchers, I am currently working on a Nanotechnology project and am exploring the process of drafting a patent to protect our innovations. Could anyone here share insights or tips on how...
02 July 2024 9,627 2 View
I am currently culturing leukocytes from TLRE filters from our blood bank. I have been struggling to culture these cells, as well as improve their viability. I was wondering if anyone would be...
16 June 2024 3,752 0 View
Have you ever seen a small number of cells, 50-100 thousand, after centrifugation as a dry pellet in an Eppendorf tube without a microscope? Are there methods to potentially stain the cells in the...
09 June 2024 6,311 0 View
formulate Phosphorus 52 and Potassium 34 in liquid
03 June 2024 7,589 2 View
I am dealing with some finicky antibodies that like to self adhere a bit in certain buffers. Working towards formulation for dosing we are trying to find good buffers that will minimize this...
30 May 2024 6,490 3 View