I am dealing with some finicky antibodies that like to self adhere a bit in certain buffers. Working towards formulation for dosing we are trying to find good buffers that will minimize this unfortunate property. To do so I need to do Dynamic Light Scattering (DLS) in a large number of different modern biologic formulation buffers to figure out which ones help minimize the self adhesion.
Unfortunately to run DLS properly a lot of non-standard (by the standards of these days) buffer properties are needed, such as refractive index and viscosity. Obviously things like the CRC handbook has a large number of critical buffer properties, but many of them are for older buffers or buffers at different concentrations than what I need.
Obviously I could measure all of these properties myself, but I am actually having trouble finding instrumentation for things like viscosity and refractive index, probably only because those are properties I have never measured before so I don't know what I even need to be looking for instrument wise.
Are there any suggestions on what is the cheapest and easiest ways to get all the parameters I need for DLS over a large number of modern buffers?
Thanks
Just use the standard values for water. The error introduced will be small.
Thanks
Would the standard values for PBS maybe be a little closer as there are salts of similar concentration dissolved in it? Obviously PBS won't absorb as strongly as some of the things like the histidine buffers nor will it have as big an impact on viscountcy, refractive index, etc... but it could be closer than water, right?
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