I am interested how can I obtain sufficient quantities of 3 to 16kb DNA fragments from genomic DNA in the fastest possible manner?
use amplitaq gold and do rePCR by making templetes from PCR products itself
I agree with Laurence, NEB Phusion beats the pack. if Phusion does not do it, the new Q5 will.
Takara LA taq is both fast and efficient.
http://www.clontech.com/DK/Products/PCR/Long_PCR/LA_Taq_DNA_Polymerase?sitex=10122:22372:US
Hi Everyone! I do my thesis about snow melting in Austrian Alps and I have some issues. I would like to create a water mask to filter out the water bodies from the study using Sentinel 2 images....
15 January 2021 4,560 1 View
I was recently invited to edit a special issue for Plants by the MDPI publisher. I know no one from my field in their editorial board. I have no experience with MDPI journals either but based on...
17 September 2020 607 8 View
Is age a moderator in this case? How do you examine its effect (i.e. higher or lower age is impacting the DV?)? Partial correlation? Other?
05 February 2020 2,193 3 View
I am planning to run pushover analysis for a lattice steel tower, but I am blocked right at the beginning since I can not define the necessary hinges. Any help is greatly appreciated!
29 March 2019 2,842 3 View
15 May 2016 659 4 View
10 May 2016 1,775 2 View
Probably Calystegia (?) species?
23 June 2015 2,430 18 View
I have started a research on trees at small sacral architecture in the rural landscape last year (within a larger work on rural green infrastructure) as a partial aspect. So far, I have evidence...
22 April 2015 8,493 17 View
03 April 2015 4,026 14 View
Hello everyone! I have a question from our mammologist group: they counted the number of anthills destroyed by bears on 89 transects (800 m each). They had a huge SD: 23.06, and the mean of...
17 February 2015 4,709 9 View
How to calculate the RMSD values for a MD simulation using MOE?
07 August 2021 0 0 View
When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.
16 June 2021 0 0 View
I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance
03 March 2021 3,568 1 View
Hi, I want to start testing pitfall trap to obtain ants samples, but I need to conduct molecular analysis on those insects. So, what kind of fluid can I use? Ethanol expires too early and I need...
03 March 2021 5,978 5 View
Hello, We would like to increase the yield of our PCR product. We are running a series of PCR reactions that is targeting ~1.1kb sequence. We begin each reaction with ~400pg of template DNA...
02 March 2021 4,029 3 View
Hi, I am trying to construct a multi-layer fibril structure from a single layer in PyMol by translating the layer along the fibril axis. For now, I am able to use the Translate command in PyMol...
02 March 2021 4,569 4 View