What is the fastest PCR (polymerase chain reaction) method for fragments of 3 to 16 kb?

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Why the absorption of n-hexane increases during UV spectrometry measurement?

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ANCOVA: When group differences emerge in variable X but age is a significant covariate, how do you interpret the Results?

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Can I base on reverse DNA sequences to perform alignment, convert to amino acids and GenBank submission?

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I can't see the ssDNA band after performing asymmetric PCR. Is there any way to do this?

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Does crude extraction using NaOH and Tris work well with Fungi?

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Are there instances where molecules with larger molecular weights exhibit greater mobility than those with smaller molecular weights?

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Sandeep RAJ R

use amplitaq gold and do rePCR by making templetes from PCR products itself

Laurence Stuart Dawkins-Hall

NEB phusion is a highly processive hot start polymerase that might do what you require:
https://www.neb.com/products/m0530-phusion-high-fidelity-dna-polymerase
https://www.neb.com/protocols/1/01/01/pcr-protocol-m0530

Peter Eck

I agree with Laurence, NEB Phusion beats the pack. if Phusion does not do it, the new Q5 will.

Mette Christiansen

Takara LA taq is both fast and efficient.

http://www.clontech.com/DK/Products/PCR/Long_PCR/LA_Taq_DNA_Polymerase?sitex=10122:22372:US