Membrane protein missing from SDS-PAGE gel?

I am trying to run an SDS-PAGE after his-tag purification of a sample. The protein of interest is an oligomerising membrane protein with an YFP tag attached. When the fractions of the sample from...

07 September 2020 3,277 6 View

What is the acceptable amount (%) of plant amplicon during ITS metabarcoding of endophytic fungi from a plant host?

During metabarcoding of endophytic fungi, many seem to use ITS primer pairs that are not (fully) selective for fungi. We experienced that such primers also amplify the ITS region of an in vitro...

09 March 2020 3,303 2 View

What is the fastest PCR (polymerase chain reaction) method for fragments of 3 to 16 kb?

I am interested how can I obtain sufficient quantities of 3 to 16kb DNA fragments from genomic DNA in the fastest possible manner?

15 September 2016 2,026 4 View

I think isotonic regression should be important in finance. Does anybody know about any applications of isotonic regression in finance?

My feeling is based on the following idea: Expected behaviour of financial data could have ordering properties. For example one may expect the increase in price of some stock in a given time...

21 June 2015 5,343 3 View

For a dynamical system x'=f(x), is there a name for a set of points y such that f(x+y)=f(x)?

15 April 2015 3,422 4 View

What is the lactate threshold or level (needed to reach during warm up) which is the most appropriate to perform the best in a 400m flat race?

Is it necessarily to reach the normal 0.9-1, 1mmol/L or higher, 3-5mmol/L? Does the actual lactate level of an athlete influence the actual physiological state?

03 June 2013 217 2 View

How optimize the Size Exclusion Chromatography?

Hello! I'll do a size exclusion chromatography, but I only have an open column, and I'll perform the peptide extraction from yeast, using buffer lysis (sodium phosphate 50 mM/NaCl 30 mM/DNAse and...

01 March 2021 2,215 2 View

No title

01 March 2021 3,905 5 View

What test should I use to compare two or three means, if data is not normally distributed but sample size is > 30 in each group?

I personally feel this as a most confusing question. As per my understanding, if sample size is > 30, and sample is skewed, we can still use paramteric approach, as it will follow Z...

01 March 2021 1,587 6 View

Is it necessary to report effect size (ES) in statistics of biomedical research given that we already report p values routinely?

Pls share your thoughts

01 March 2021 7,330 4 View

Why there are two peaks while using the mie plot of Silver nanoparticle?

Hi, I am trying to learn the theoretical modelling of silver nanoparticle using the Mie scattering theory. After that, I discovered a software named Mieplot developed by Philip Laven, where it...

25 February 2021 7,493 6 View

How to calculate Z (the number of molecules in a unit cell) for calculating theoretical density?

I am trying to calculate the theoretical density of my ceramics materials. I use the formula below to calculate the theoretical density: Theoretical density = (Molecular weight x No. of molecules...

24 February 2021 3,823 2 View

How to handle studies which report perfect effect sizes in a meta-analysis using Pearson's r?

I'm in the process of updating a meta-analysis and in one of the new studies, effect sizes are reported as Tau-U. Two of the effect sizes are reported as Tau-U = 1.00. I am using Andy Field's...

24 February 2021 2,265 3 View

Sample size determination for non-parametric tests?

How do I run a a priori sample size determination for non-parametric tests, like the Mann-Whitney U-test and the Kruskall-Wallis or other non-parametric tests that accord with the unpaired t-test...

24 February 2021 166 6 View

How to determine a priori sample size for t-test with unequal means?

I need to conduct a a priori sample size determinations for t-test with unequal means, but I don't know the sizes of the groups nor the ratio of both groups... Can I do a sample size determination...

24 February 2021 8,289 3 View

What are alternatives to factor analysis for a small sample?

My sample size is too small to conduct a factor analysis. I created my own psychometric scale for measuring Effectiveness of Support, which contains 38 items (4 sub-scales) Im really struggling...

23 February 2021 9,287 12 View