I am trying to find fluorescence of dyes in physiological pH, but I am not certain about the exc and em wavelengths of phosphate buffer.
Hello! I have conducted a series of experiments for drug binding of a certain compound to HSA (serum albumin) such as fluorescence, UV-Vis, etc. Is it alright to publish my results without an...
09 October 2017 362 6 View
Can someone explain in simple terms how a Matching algorithm (used in DOCK, FLOG, LibDock and SANDOCK) work.
02 March 2017 8,717 0 View
As I can observe from various papers, what can be the possible reason why NONOates are synthesized with very low temperature conditions? Is it because the multi-nitrogen species can react violently?
02 March 2016 1,917 0 View
Hi there. What method can I employ if I want to deprotec a Boc-protected secondary amine without using acid? or simply put some mild conditions for Boc deprotection without acid reagents.
02 March 2016 3,452 6 View
I am currently interested in biophotonics and I want to apply it to biochemical samples though I do not know how to begin. Would you help me brainstorm with this one?
31 December 2015 437 1 View
What computational chemistry tool can be best for this time of system, especially that phosphatidylserine and phospatidylethanolamine have interactions in the lipid bilayer?
31 December 2015 3,713 4 View
I am using Rhodamine6G as gain medium and silver nanoparticles as scatterers on a microscope slide and laser input 532 nm comes from above.
09 August 2024 9,894 2 View
Does anyone tried to do nucleofection with AMAXA by Lonza with lower than recommended amount of buffer in the cuvettes (100 ul)?
07 August 2024 4,616 0 View
Currently, when I run SDS-PAGE, I don't see any bands at all, even though I used the same material just a day ago and it worked fine.... In our lab, we dilute the 10X running buffer to 1X and...
06 August 2024 5,373 2 View
After immunohistochemistry of previously fixed in PFA and EtOH and then frozen 20 μm sections of zebrafish brain, DAPI staining is very weak (right) compared to the same sections stained without...
05 August 2024 9,637 2 View
Hello everyone, I'm encountering an issue with my electrochemical impedance spectroscopy (EIS) measurements and would appreciate some insights. Experimental Setup: Electrodes: Gold interdigitated...
05 August 2024 3,783 2 View
Are there any fluorescently labeled anti-Alpaca secondary antibodies raised in Donkey? So far I have only been able to find anti-Alpaca secondaries raised in Goat. Or is this not possible due to...
04 August 2024 4,255 1 View
Hello. I am working on ROS production of two systems: system A is cerium oxide and hydrogen peroxide, system B is cerium oxide nanoparticle, hydrogen peroxide and potassium bromide. I did some...
04 August 2024 5,974 3 View
How to calculate the molar proportions of the oxides from the XRF analyses?
02 August 2024 2,565 2 View
Is it the "elution buffer" or the "dialysis buffer"? Note: I'll be using NanoDrop OneC
01 August 2024 967 3 View
Dear All, I am trying to transfect a pCDNA3.1 vector containing my gene of interest. The purpose is to figure out the localization of the protein of interest. I have fused the protein with GFP on...
31 July 2024 9,892 4 View