Drug binding to HSA without FT-IR?

Hello! I have conducted a series of experiments for drug binding of a certain compound to HSA (serum albumin) such as fluorescence, UV-Vis, etc. Is it alright to publish my results without an...

09 October 2017 258 6 View

How does a Matching Algorithm work in molecular docking?

02 March 2017 8,575 0 View

Why is the synthesis of NONOate normally carried out at temperatures below zero?

As I can observe from various papers, what can be the possible reason why NONOates are synthesized with very low temperature conditions? Is it because the multi-nitrogen species can react violently?

02 March 2016 1,824 0 View

Any suggestion on Boc deprotection without using acid?

Hi there. What method can I employ if I want to deprotec a Boc-protected secondary amine without using acid? or simply put some mild conditions for Boc deprotection without acid reagents.

02 March 2016 3,307 6 View

What research topics in biophysical or bioanalytical chemistry that can be carried out by a simple undergraduate student like me?

I am currently interested in biophotonics and I want to apply it to biochemical samples though I do not know how to begin. Would you help me brainstorm with this one?

31 December 2015 338 1 View

What method is best if one is trying to predict the bond formation of metals with phosphatidylserine and phosphatidylethanolamine in cell membranes?

What computational chemistry tool can be best for this time of system, especially that phosphatidylserine and phospatidylethanolamine have interactions in the lipid bilayer?

31 December 2015 3,608 4 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

Buffer to disrupt platelet alpha granules for PF4 quantification?

Hello! I want to quantify by ELISA the secreted (from platelets poor plasma) and the non-secreted (from platelet lysate) PF4 before and after TRAP stimulation. I will use the ELISA from R&D...

03 March 2021 1,499 2 View

Buffer Exchange using column packed with sephadex G-25 effect on protein concentration?

Hello, If i am doing a buffer exchange for an antibody of 1mg/mL, does the elution lose protein in the process of buffer exchange? For example, if i flow through 500 uL of 1mg/mL sample, and...

03 March 2021 6,299 3 View

Is There Any Feasible Method To Test Fluorescent Compounds Other Than Fluorescence Spectrometers ??

Is There Any Feasible Method To Test The Efficiency Of Fluorescent Compounds Other Than UV Spectrometers ? Suggestions Would Be Appreciated !

02 March 2021 5,785 3 View

How optimize the Size Exclusion Chromatography?

Hello! I'll do a size exclusion chromatography, but I only have an open column, and I'll perform the peptide extraction from yeast, using buffer lysis (sodium phosphate 50 mM/NaCl 30 mM/DNAse and...

01 March 2021 2,215 2 View

Why are my bands weird in this Agarose gel?

Can someone please give me some possible things that could go wrong? Here is my recipe: 0.5g Agarose 50 mL of TAE 1x 1 uL ethyl bromide. Gel was run at 100V for 1 hour. The buffer used is also TAE.

01 March 2021 9,952 3 View

Why might my GFP tag be dissociating from my plasmid after transfection?

I have a set of stably transfected cell lines all transfected with plasmids containing GFP tags on the C terminus. During a western blot using anti-GFP antibody, one of my plasmids has dissociated...

01 March 2021 9,310 4 View

Which fluorescent dye to use for measuring total ROS production?

Is DCFDA appropriate for measuring total ROS production, or can it only detect certain types of ROS? If I want to measure total ROS production, which dye should I use?

01 March 2021 9,447 3 View

How to accurately quantify chitin?

Hi all, I have been working on the quantification of chitin in insect samples and feed for a while but I can notice that the quantification methods (e.g. exposure to HCl, NaOH, and weighing sample...

01 March 2021 1,843 2 View

What do I do with DLS peaks that are from the buffer?

I'm looking at the aggregation of my protein sample using DLS. Unfortunately, my buffer (20mM HEPES) also results in a set of peaks. These are at approximately 1 and 1000 d.nm. The lowest peak...

01 March 2021 9,015 2 View