What is the effect of these two factors (EDTA and sonication process) on my DNA?

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Omar Gonzalez-Ortega

I am not familiar with DNA, I only know that EDTA has to do with having single stranded DNA. What I know is that stability issues could arise upon DNA adsorption. DNA will be cleaved under extreme sonication.

Muhammad Nur Hasan

So, when I heating up again.. Will it be good conformation (the DNA form a hairpin structure)??

Thanks

Adam B Shapiro

Long DNA molecules can be broken by sonication. It is one way to prepare random DNA fragments from genomic DNA. Find a way to purify your NPs after conjugation that does not require pelleting.

Thank you Prof. Adam B. Shapiro