11 Questions 10 Answers 0 Followers
Questions related from Muhammad Nur Hasan
Previously, I made Pb(NO3) stock in miliQ water and diluted into buffer. But I tried different way yesterday. I prepared 100 uM of Pb(NO3) in buffer 10 mM TA containing 50 mM NaCl, pH: 7.0-7.4 The...
13 February 2020 6,114 2 View
Normally, I have fresh prepared TAE/TBE buffer. But sometimes I see one of my colleagues who re-use it max. 3 times, Is it corret or not? Does it have any effect to the result? Thanks
20 March 2019 3,006 3 View
I want to chelate metal ion using EDTA. I saw in blood tube for anti-coagulant, they use K2-EDTA. But I have disodium salt EDTA in my lab (mw: 372.24 g/mol). So, which one is better? Thank you.
07 February 2018 1,190 5 View
I have buffer containing sucrose solution for pre-treatment and washing. When I sterilized it in autoclave, sometimes the color will be changed (almost brown).
31 January 2018 505 2 View
I have buffer was stored more than 6 months. Sometime the pH is changed. Suppose, what causes of the pH can be changed in the stored buffer? Thanks.
22 January 2018 1,711 3 View
1. I dissolve my DNA in PBS 1X containing EDTA 0.5 M. 2. After conjugated with gold nanoparticles (AuNps), I centrifuge it but the pellet cannot be re-dispersed. So, I sonicate until...
08 January 2018 9,352 4 View
When I made 10% SDS (w/v), it was difficult to dissolve. The powder were still staying in solution (milliQ water 100 ml). Eventhough I mixed by stirrer and heat up at 25 C. After that, I sonicated...
20 December 2017 1,057 3 View
Which one good buffer for pre-treatment of conjugate pad in Lateral Flow Assay: 1) Tris-acetate containing NaCl and 8% sucrose 2) Tris-HCl containing NaCl and 0.25% Triton X-100 3) Sodium borate...
08 June 2017 7,124 3 View
I want to make like these figures, but my DNA sequences is different.
25 February 2017 4,737 0 View
The biotinylated capture DNA-streptavidin complexes were then dispensed onto 7 test lines made up of NC membrane.
19 January 2017 3,237 3 View
If we want to fabricate LFA or uPaD, we need to prepare AuNPs-DNA reporter probe or using AuNPs-Antibody as a label (colorimetri). Other solution that we have to prepare is Bovin Serum Albumin...
01 January 1970 8,627 1 View
