What is the consensus motif for Disabled-2 (Dab2) binding? I am familiar with the NPXY motif but I am wondering if there are any others.
Just a generic question, what is the average half-life of a typical membrane protein? If you have a review off hand that could be sent to me, that would be greatly appreciated.
03 April 2019 384 3 View
I have an Orion Versa Star Pro Multi-Meter and I need to get data off of it into Microsoft Excel. We are currently using Hyper Access as a logging software but this is not meeting our needs. We...
31 December 2018 6,246 0 View
I am looking for an Olympus Cx43 model microscope that has been used or was a demo model. I would also settle for other earlier models of Olympus microscopes. I need a 100X objective, a camera...
10 November 2018 4,188 8 View
Can food grade CO2 be used in place of medical grade CO2 for a cell culture incubator? Our lab is running out of medical grade CO2 and all we have on hand is food grade. We can't order more...
05 June 2018 433 0 View
Hi, I need a good inhibitor for CDC42. I am new to working with this protein and wonder if anyone knows what inhibits it well. Thank you! Chuck
03 April 2018 3,973 10 View
I am looking for a dominant negative Wiskott Aldrich Syndrome Protein Mutant. I would prefer if it has an mCherry tag. Our lab would pay for shipping and acknowledge your contributions to any...
02 March 2018 6,417 0 View
I am trying to delete 30nt regions of a plasmid but have been unsuccessful in my PCR using overlap extension PCR. My primers range in size between 23 and 36 bp in length. I fear that my deletion...
11 December 2017 4,905 6 View
I am trying to purify DNA from a gel but yields are seemingly too low. I have loaded ~500ng of DNA onto my gel, run it, and cut it out and purified it using a Quigen Gel Extraction kit. I am only...
11 December 2017 6,562 13 View
Hello, I am relatively inexperienced with certain molecular biology techniques. I have a gene that has been cloned into a plasmid for me. I would like to leave the N and C Terminal domains intact...
10 November 2016 1,185 2 View
Hello, I am attempting to use Quikchange Mutagenesis to introduce a single nucleotide mutation into a circular plasmid. I have run the mutagenesis protocol in a thermal cycler with the following...
05 June 2016 2,722 7 View
I have carried out MFC experiments on three different volumes, 50, 500 and 1000 mL of wastewater. Results after MFC treatment shows that TDS and EC are more in larger volumes of water i.e. TDS and...
09 August 2024 9,621 0 View
Hello everyone! I observed in my culture (htert-RPE1 cells) an orange- red particle at the bottom of the dish. It is visible to the naked eye as a very very small red dot. Could it be a...
09 August 2024 2,824 3 View
I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...
07 August 2024 7,535 4 View
Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...
07 August 2024 8,106 4 View
Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...
06 August 2024 641 2 View
Hi! So i attempted to understand a novel protein behavior towards heat application by analyzing its secondary structure change. I subjected the protein to a thermal denaturation analysis using...
06 August 2024 1,989 3 View
Hello, Can anyone provide me with the absorption coefficient of methane gas at 7.7 um? Any reference?
06 August 2024 980 5 View
I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...
04 August 2024 9,913 7 View
I have protein-membrane simulations (PDB, PSF, DCD) and have noticed that water molecules near the protein are not visible in the simulations. How can I fix this issue? Is there a way to place the...
04 August 2024 1,200 2 View
Hi, I am isolating monocytes from the bone marrow using the Mouse Monocyte EasySep kit. I want to treat these cells and monitor expression of specific markers over the course of 10 days. I will...
04 August 2024 7,282 2 View