What is the average half-life of a typical membrane protein?

Just a generic question, what is the average half-life of a typical membrane protein? If you have a review off hand that could be sent to me, that would be greatly appreciated.

03 April 2019 197 3 View

Experience connecting Versa Star Pro Multi-Meter to Software?

I have an Orion Versa Star Pro Multi-Meter and I need to get data off of it into Microsoft Excel. We are currently using Hyper Access as a logging software but this is not meeting our needs. We...

31 December 2018 6,094 0 View

Where can I find used or demo microscopes?

I am looking for an Olympus Cx43 model microscope that has been used or was a demo model. I would also settle for other earlier models of Olympus microscopes. I need a 100X objective, a camera...

10 November 2018 4,072 8 View

Food grade vs. medical grade CO2 for cell culture incubator?

Can food grade CO2 be used in place of medical grade CO2 for a cell culture incubator? Our lab is running out of medical grade CO2 and all we have on hand is food grade. We can't order more...

05 June 2018 243 0 View

What is a good CDC42 Inhibitor?

Hi, I need a good inhibitor for CDC42. I am new to working with this protein and wonder if anyone knows what inhibits it well. Thank you! Chuck

03 April 2018 3,829 10 View

Does anyone have a dominant negative Wiskott Aldrich Syndrome Protein Mutant they would be willing to share?

I am looking for a dominant negative Wiskott Aldrich Syndrome Protein Mutant. I would prefer if it has an mCherry tag. Our lab would pay for shipping and acknowledge your contributions to any...

02 March 2018 6,303 0 View

Primer size for deletion mutagenesis using overlap extension PCR?

I am trying to delete 30nt regions of a plasmid but have been unsuccessful in my PCR using overlap extension PCR. My primers range in size between 23 and 36 bp in length. I fear that my deletion...

11 December 2017 4,766 6 View

What is the minimum amount of DNA required to be loaded on a gel for a gel extraction/purification?

I am trying to purify DNA from a gel but yields are seemingly too low. I have loaded ~500ng of DNA onto my gel, run it, and cut it out and purified it using a Quigen Gel Extraction kit. I am only...

11 December 2017 6,436 13 View

How do I cut out a section of a plasmid?

Hello, I am relatively inexperienced with certain molecular biology techniques. I have a gene that has been cloned into a plasmid for me. I would like to leave the N and C Terminal domains intact...

10 November 2016 1,069 2 View

Can someone help me with mutagenesis troubleshooting?

Hello, I am attempting to use Quikchange Mutagenesis to introduce a single nucleotide mutation into a circular plasmid. I have run the mutagenesis protocol in a thermal cycler with the following...

05 June 2016 2,608 7 View

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02 March 2021 3,060 3 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Why might my GFP tag be dissociating from my plasmid after transfection?

I have a set of stably transfected cell lines all transfected with plasmids containing GFP tags on the C terminus. During a western blot using anti-GFP antibody, one of my plasmids has dissociated...

01 March 2021 9,310 4 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Precipitate after adding laemmli buffer to protein solution containing Potassium Acetate?

Hi, I am running a size exclusion chromatography experiment with a buffer containing Potassium Acetate as a salt. I analyse these fractions through SDS-PAGE. After boiling my SEC fractions in...

01 March 2021 2,622 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

What is the most suitable server for predicting protein structures in bacteria?

I have used the i-Tasser several times, however it has been unavailable for several days. I tried the swiss-model, but the output was not very pleasant due to the model used. Is there any other...

28 February 2021 4,521 3 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View

Are protein samples in SDS compatible with ELISA?

I have used an AllPrep DNA/RNA/Protein Mini Kit QIAGEN kit to extract RNA and protein from my samples. At the end of the protein purification, I resuspend my protein in 5% SDS. Will these samples...

28 February 2021 7,370 3 View