What is the average half-life of a typical membrane protein?

Just a generic question, what is the average half-life of a typical membrane protein? If you have a review off hand that could be sent to me, that would be greatly appreciated.

03 April 2019 197 3 View

Where can I find used or demo microscopes?

I am looking for an Olympus Cx43 model microscope that has been used or was a demo model. I would also settle for other earlier models of Olympus microscopes. I need a 100X objective, a camera...

10 November 2018 4,072 8 View

Food grade vs. medical grade CO2 for cell culture incubator?

Can food grade CO2 be used in place of medical grade CO2 for a cell culture incubator? Our lab is running out of medical grade CO2 and all we have on hand is food grade. We can't order more...

05 June 2018 243 0 View

What is a good CDC42 Inhibitor?

Hi, I need a good inhibitor for CDC42. I am new to working with this protein and wonder if anyone knows what inhibits it well. Thank you! Chuck

03 April 2018 3,829 10 View

Does anyone have a dominant negative Wiskott Aldrich Syndrome Protein Mutant they would be willing to share?

I am looking for a dominant negative Wiskott Aldrich Syndrome Protein Mutant. I would prefer if it has an mCherry tag. Our lab would pay for shipping and acknowledge your contributions to any...

02 March 2018 6,303 0 View

Primer size for deletion mutagenesis using overlap extension PCR?

I am trying to delete 30nt regions of a plasmid but have been unsuccessful in my PCR using overlap extension PCR. My primers range in size between 23 and 36 bp in length. I fear that my deletion...

11 December 2017 4,766 6 View

What is the minimum amount of DNA required to be loaded on a gel for a gel extraction/purification?

I am trying to purify DNA from a gel but yields are seemingly too low. I have loaded ~500ng of DNA onto my gel, run it, and cut it out and purified it using a Quigen Gel Extraction kit. I am only...

11 December 2017 6,436 13 View

How do I cut out a section of a plasmid?

Hello, I am relatively inexperienced with certain molecular biology techniques. I have a gene that has been cloned into a plasmid for me. I would like to leave the N and C Terminal domains intact...

10 November 2016 1,069 2 View

Can someone help me with mutagenesis troubleshooting?

Hello, I am attempting to use Quikchange Mutagenesis to introduce a single nucleotide mutation into a circular plasmid. I have run the mutagenesis protocol in a thermal cycler with the following...

05 June 2016 2,608 7 View

How can I get a membrane IMPERMEABLE dye into a vast number of cells?

I want to get a membrane IMPERMEABLE dye into a large number of HeLa Cells in culture. What are some techniques to introduce a membrane impermeable dye into cells?

01 February 2016 2,586 3 View

error in running a model in Abaqus

Excessive rotation of nodes in node set ErrNodeExcessRotation-Step1

17 August 2021 0 0 View

error in modelling model in Abaqus

Excessive rotation of nodes in node set ErrNodeExcessRotation-Step1

17 August 2021 0 0 View

MGL tools

20 June 2021 0 0 View

i have some problem with chtMultiRegionSimpleFoam

--> FOAM FATAL ERROR: Maximum number of iterations exceeded From function Foam::scalar Foam::species::thermo::T(Foam::scalar, Foam::scalar, Foam::scalar, Foam::scalar (Foam::species::thermo::*)(Foam::scalar, Foam::scalar) const, Foam::scalar (Foam::species::thermo::*)(Foam::scalar, Foam::scalar) const, Foam::scalar (Foam::species::thermo::*)(Foam::scalar) const) const [with Thermo = Foam::hConstThermo >; Type = Foam::sensibleEnthalpy; Foam::scalar = double; Foam::species::thermo = Foam::species::thermo >, Foam::sensibleEnthalpy>] in file /home/ubuntu/OpenFOAM/OpenFOAM-4.1/src/thermophysicalModels/specie/lnInclude/thermoI.H at line 66. FOAM aborting #0 Foam::error::printStack(Foam::Ostream&) at ??:? #1 Foam::error::abort() at ??:? #2 Foam::heRhoThermo >, Foam::sensibleEnthalpy> > > >::calculate() at ??:? #3 Foam::heRhoThermo >, Foam::sensibleEnthalpy> > > >::correct() at ??:? #4 ? at ??:? #5 __libc_start_main in "/lib/x86_64-linux-gnu/libc.so.6" #6 ? at ??:?

24 March 2021 0 0 View

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10?

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10? Since, the growth curve from day 2 to 10 won't be like the "Logistic curve" it might not follow logistic...

03 March 2021 1,401 3 View

What is the production process of CaFe ferroalloys?

I looking for ferro calcium production process

03 March 2021 1,755 4 View

Resistor definition in Piezoelectric bimorph with ANSYS workbench

Hello, good day! I am trying to simulate similar bimorph piezoelectric harvester with below commands. However, the output power doesn't change with resistor value. Please help to find the reason. ET,10,CIRCU94,0 !SET UP RESISTOR R,1,100000 !RESISTANCE VALUE TYPE,10 !SET ELEMENT TYPE E,1,2 !CREATE RESISTOR BETWEEN NODES 1 AND 2 Thanks in advance Regards,

02 March 2021 0 0 View

No title

02 March 2021 3,060 3 View

How to calculate BREQ 3 questionnaire?

how to calculate BEHAVIOURAL REGULATION IN EXERCISE QUESTIONNAIRE BREQ 3 ,which contain 24 questions and each question has 5 answers (0- Strongly disagree , 1-disagree, 2- Neither agree nor...

02 March 2021 811 3 View

Does Napabucasin soluble in dichloromethane and in ethanol?

I need to prepare a solution of Napabucasin (inhibitor of cancer cell stemness) in dichloromethane. The drug is expensive, so maybe somebody already tried to do it. If it is soluble, what is the...

02 March 2021 9,945 3 View