By using thermosensitive chitosan-beta glycerophosphate hydrogel as the scaffold, do we still need to add beta-glycerophosphate in the differentiation media?
Our western blotting for tyrosine hydroxylase consistently shows double bands (50-70 kDa) in murine hearts using the #AB152 antibody from Sigma-Aldrich. Has anyone else encountered this issue? If...
30 May 2024 4,308 2 View
So I'm trying to determine what radical this is captured with DMPO. How do I determine and calculate what radical this is? Thanks!!
06 April 2024 7,084 3 View
In plinian eruptions there are pyroclastic density currents PDCs which are generated directly from the collapse of the eruption column, this collapse zone has a range of heights and is very useful...
06 September 2023 3,848 0 View
I'm doing electrochemical analysis with modified graphite felt as the working electrode and Fe(CN) as the electrolyte. In my CV plot, △Ep became larger, but Ipc/Ipa became close to 1. Is it...
23 December 2022 5,869 4 View
I am using Google Earth Pro to get some images for maps in my research area, and also to get some coordinates of my study area, and I am required to cite it. But I am not sure how the citation may...
19 December 2022 9,506 2 View
Is it common for surface modification to increase the standard deviation of electrochemical measurements ? I read several articles but did not investigate this issue. I'd like to know if there are...
24 June 2022 2,297 2 View
I tried to predict the structure of protein complex by alphafold2 using AlphaFold2_complexes (https://colab.research.google.com/github/sokrypton/ColabFold/blob/main/AlphaFold2_complexes.ipynb) To...
18 May 2022 4,581 0 View
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17 January 2022 9,086 3 View
My PhD aims to explore the role of leadership and motivation in sustaining volunteer efforts, particularly in the emergency, health and welfare sectors. I'm looking for expert judges to help...
17 January 2022 245 3 View
Dear colleague, I am testing on formulation of cationic lipid nanoparticles. The lipid compostiin is DOTAP: DSPC: Cholesterol: PEG2000 DSPE=50: 10: 39: 1. The buffers of 50 mM sodium acetate at...
11 December 2021 2,800 1 View
We are working on biopolymeric hydrogels. Our system is highly viscous and sticky, and the gel formed are high in strength. We are unable to use pH electrode and pH strip. Please suggest an easy...
30 July 2024 942 2 View
My final research is about making chitosan/pva hydrogel for wound healing using gelatin as crosslinker. Last month, i made the thin film hydrogel and it turn well. It was okay. Last week, i make...
28 July 2024 2,794 6 View
I'm working with a hydrogel sample and I'd like to perform XRD analysis. Can anyone offer guidance on preparing the hydrogel for the XRD instrument? Specifically, I'm unsure about the best method...
20 July 2024 3,611 4 View
Can the kit from Invitrogen™ LIVE/DEAD™ Cell Imaging Kit (488/570) be used on fixed cells on a scaffold?
08 July 2024 7,914 3 View
I have primary breast cells embedded in the hydrogel. When stained with EpCAM without the hydrogel, the staining was correctly localized to the membrane as expected! However, after embedding in...
28 June 2024 353 1 View
"What is the most accurate method and model for evaluating the effective mechanical and viscoelastic properties due to the inclusion of multiple types of particles in a viscous material, and which...
16 June 2024 9,139 1 View
I want to see if there are any technology out there that will allow me to do a cell count inside of a hydrogel (the hydrogel is inside an insert). We currently have hydrogel inside an insert and...
12 June 2024 6,390 1 View
Why is there no peak shift in FTIR-ATR after the amine-modified polystyrene nanoplastics (PS-NH2) adsorption onto the NIPAAM/CMC hydrogel materials? I have repeated the tests, but I have gotten...
09 June 2024 9,007 6 View
I have been making hydrogels regularly using 10X DMEM (phenol red indicator), Ultrapure water, 1N NaOH, and 3.41 mg/ml Collagen (suspended with acetic acid). We have used the same calculations for...
29 May 2024 4,655 0 View
A uniform mixture of chitosan and gelatin is made by dissolving in acetic acid, after that the mixture is transferred to a plastic petri plate kept at -20 C for 24h and then lyophilized. But i am...
29 May 2024 7,367 4 View