Does anyone have experience using High Molecular Weight DNA Extraction Kits for extracting DNA from plant leaves?

Hi there, I'm looking for a High Molecular Plant DNA Extraction Kit for subsequent Nanopore sequencing. Does anyone have experience using commercially available kits? Any...

03 January 2021 9,487 4 View

How can I decrease surface roughness in selective laser melting?

Recently, I study master degree in Taiwan. And my research topic is about metal 3-d printing with in718. I am required to decrease surface roughness in overhanging structure or inclined cube. Is...

02 November 2020 8,127 3 View

Final stage process development for lipid nanoparticles?

Dear Colleagues, To design a final stage process development for lipid nanoparticle formation using microfluidics, what aspects should be taken into account? Thank you for your...

09 August 2020 750 3 View

How to calculate drug compliance when topical administration?

Hi everyone, I am currently design an experiment about compare patients' drug compliance. The drug is used topically twice daily on the wound. We would provide diary card for subject to note the...

19 February 2020 6,891 1 View

Which software should a researcher working on continuous gas lift optimization use? PROSPER (PETEX) or PIPESIM (Schlumberger)?

I want to know which of the two softwares will be handy considering working on continuous gas lift optimization.

14 January 2020 8,901 2 View

Can I use T-overhang TOPO cloning vector for blunt end PCR product?

Hi, I know there are T-overhang and blunt end TOPO cloning vectors available from different vendors. Does anyone know if I can join blunt end PCR product with T-overhang TOPO vector? or I must...

30 May 2019 6,033 1 View

Is it possible to estimate the biomass of a crop knowing only the yield per acre per year?

I am trying to estimate the carbon input (of vegetal residue) to an agricultural land, based on the historical records of the yield. However, the yield (lb per acre) is the only information I...

14 April 2019 7,370 3 View

Need I use western blot to confirm the untargeted LC-MS/MS result?

Hi, I had two samples (with or without treatment) sent for untargeted proteomics analysis through mass spectrometry. We found there is a change in amount of an enzyme. I want to know if I need...

03 May 2018 1,719 3 View

Can we send published paper to those who request for it?

I got many requests for full papers but I wonder if I can offer them since the copyright have been released to the publisher when paper was published.

07 March 2018 6,060 17 View

Has anybody used Oligofectamine for co-transfection of siRNA and Plasmid DNA?

I am planning to co-transfect siRNA and Plasmid DNA and I have only Oligofectamine with me. Though Oligofectamine is recommended only for siRNA transfection I was wondering if Plasmid DNA can also...

17 April 2017 8,129 2 View

How to model Type 1 collagen structure for finite element analysis ?

I have started a project in which stress distribution in Type 1-collagen scaffold is to be modelled. I am thinking of using finite element analysis to model the structure and the loading...

07 February 2021 5,487 3 View

Can I polymerize NIPPAm with PEGmethacrylate or only with PEGacrylate?

I want to perform the synthesis of a hydrogel and I want to know if I can polymerize NIPPAm with PEGmethacrylate or only with PEGacrylate?

02 February 2021 2,106 6 View

How can solve/disperse silicon nanoparticles in water?

I want to add silicon nanoparticles to my hydrogel, but when I sonicated these particles with water to disperse them in water, they agglomerated on the water's surface. How can I disperse them in...

26 January 2021 213 4 View

Anyone know how to dilute strong hydrogel structure?

I am working on PVA-GMA hydrogel that is a really strong polymeric structure like rubber. Because I am in the middle of analysis, it is difficult to dissolve the gel using a common solvent. I...

20 January 2021 3,501 2 View

How can I stain 3d printed cells in hydrogel without damaging the gel?

I tried staining my cells in a gel using standard protocols with 4PF% for fixation and triton-X or saponin for permeabilization but the gel always gets damaged throughout the steps, and at the end...

17 January 2021 4,170 1 View

Why Selenium nanoparticles synthesized by PEG 200 could not be scaled up ?

I could not synthesize Selenium nanoparticles by PEG 200 at up-scale ( more than 100 ml) as it got aggregated while at small amounts it works fine , I work with 850 PPM using selenium powder in...

08 January 2021 10,049 1 View

How can I take an electrical conductivity test from a nanofibrous scaffold?

I have a nanofiber scaffold for tissue engineering. When I did the electrical conductivity test, the ohmmeter reports a wide range of values (from 300 kOhm to 20 MOhm). I placed the scaffold...

06 January 2021 2,232 1 View

Does soft algniate hydrogel influence calcium concentration of a solution (cell culture medium)?

05 January 2021 6,066 4 View

After cellulose is regenerated in water, a cellulose hydrogel is formed. I want to know if there is crystallinity inside the the hydrogel?

After cellulose is dissolved and regenerated in water, a regenerated cellulose hydrogel is formed. I want to know if there is crystallinity inside the hydrogel, or it will not crystallize until it...

04 January 2021 7,466 4 View

When measuring the resistance of hydrogel, how to solve the data instability?

When I was measuring the hydrogel resistance, I found that the data was not very stable because of the wire or the DIRECT current

02 January 2021 3,205 2 View