I tried to predict the structure of protein complex by alphafold2 using AlphaFold2_complexes (https://colab.research.google.com/github/sokrypton/ColabFold/blob/main/AlphaFold2_complexes.ipynb)

To test this is work, I used the FASTA sequences from IL-33/ST2, a known binary complex from PDB.

However, this error was presented:

Call MMseqs2 to get MSA for each gene

running mmseqs2 (use_env=True filter=True)

COMPLETE: 100%

300/300 [elapsed: 03:03 remaining: 00:00]

---------------------------------------------------------------------------

TypeError Traceback (most recent call last)

in () 36 print("pairs found:",len(msa)) 37 ---> 38 msa, deletion_matrix = pipeline.parsers.parse_a3m(a3m_lines[0]) 39 msas.append([seq+"-"*Ls[1] for seq in msa]) 40 deletion_matrices.append([mtx+[0]*Ls[1] for mtx in deletion_matrix])

TypeError: cannot unpack non-iterable Msa object

Can anyone tell me how to fix this problem?