Recently ,we decide to extract cancer stem cell from primmery cancer cells. for this aim can we use the non-treated flask. on the other hand what is the difference of non-treated flask with Ultra-Low attachment cell culture flasks?
To add to the previous answer: While non-tissue culture treated plasticware is not optimized to support high cell attachment, it does not completely prevent cell attachment for all cell lines. It has a hydrophobic surface that can support some level of non-specific binding of attachment proteins and so it can consequently support some level of cell attachment. Ultra-low attachment surface is optimized to prevent binding of cell attachment proteins through hydrophobic or ionic interactions. If you plan to use non-tissue culture treated plasticware for cancer stem cell culture, you may want to try to coat its surface with sterile 1% agarose in water, which can further decrease the chance of cell attachment.
Ultra-Low attachment cell surface is a hydrophilic, neutrally charged coating covalently bound to a polystyrene vessel surface. The hydrogel inhibits specific and nonspecific immobilization, forcing cells into a suspended state, enabling 3D spheroid formation. For your aim, it is better to use ultra-low attachment cell culture flask.
To add to the previous answer: While non-tissue culture treated plasticware is not optimized to support high cell attachment, it does not completely prevent cell attachment for all cell lines. It has a hydrophobic surface that can support some level of non-specific binding of attachment proteins and so it can consequently support some level of cell attachment. Ultra-low attachment surface is optimized to prevent binding of cell attachment proteins through hydrophobic or ionic interactions. If you plan to use non-tissue culture treated plasticware for cancer stem cell culture, you may want to try to coat its surface with sterile 1% agarose in water, which can further decrease the chance of cell attachment.
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